Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Proliferation and Metastasis of Osteosarcoma Cells by Targeting c-Met and SOX4 via miR-34a/c-5p and miR-449a/b

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Zhenyu SunBo Chen

Abstract

BACKGROUND Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a functional long non-coding RNA involved in many biologic processes. The study was aimed to explore the functional roles of MALAT1 in osteosarcoma progression. MATERIAL AND METHODS A total of 76 osteosarcoma tissues and paired adjacent non-tumor tissues were collected from surgical resection. MALAT1, miRNAs, and genes mRNA expression levels were detected using quantitative real-time PCR (qRT-PCR). Protein expression level, cell proliferation, migration, and invasion were assessed using western blot, Cell Counting Kit-8 (CCK-8), wound-healing assay, and Matrigel invasion assay respectively. The target relationships among miRNAs, MALAT1, and mRNA were detected via dual-luciferase reporter assay. RESULTS We found that MALAT1 was frequently upregulated in osteosarcoma samples and cell lines and a high level of MALAT1 predicted poor overall survival in osteosarcoma patients. Knockdown of MALAT1 inhibited proliferation, migration, and invasion of osteosarcoma cells. Further study showed a positive correlation between MALAT1 and c-Met or SOX4 expression. Mechanistic investigations demonstrated that MALAT1, as a competing endogenous RNA (ceRNA), regulated os...Continue Reading

Citations

Mar 29, 2020·Frontiers in Cell and Developmental Biology·Hong ZhanJun Lin
Feb 23, 2020·Frontiers in Cell and Developmental Biology·Xin LiuZujiang Yu
Nov 24, 2020·The International Journal of Biochemistry & Cell Biology·Ugo Ala
Oct 30, 2020·Cancer Management and Research·Shijian FuQuanzhong Liu
Aug 13, 2021·Cell Death Discovery·Yan LiuZuozhang Yang
Dec 18, 2021·Chemical Biology & Drug Design·Faezeh MalakotiForough Alemi

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Methods Mentioned

BETA
surgical resection
transfection
PCR
Assay

Software Mentioned

SPSS18

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