Long non-coding RNA MIAT promotes breast cancer progression and functions as ceRNA to regulate DUSP7 expression by sponging miR-155-5p

Oncotarget
Tian LuanLihong Wang

Abstract

Long non-coding RNAs (lncRNA) have been reported as key regulators in the progression and metastasis of breast cancer. In this study, we found that the lncRNA myocardial infarction associated transcript (MIAT) expression was upregulated in breast cancer in The Cancer Genome Atlas (TCGA) data sets. We validated that MIAT was higher in breast cancer cell lines and advanced breast tumors than in normal controls. And MIAT overexpression associated with TNM stage and lymphnode metastasis. Knockdown MIAT inhibited breast cancer cell proliferation and promoted apoptosis. Also MIAT downregulation suppressed epithelial-mesenchymal transition (EMT) and decreased migration and invasion in MDA-MB-231 and MCF-7 breast cancer cell lines. More importantly, knockdown MIAT inhibited tumor growth in vivo. Our results suggested that MIAT acted as a competing endogenous RNA (ceRNA) to regulate the expression of dual specificity phosphatase 7 (DUSP7) by taking up miR-155-5p in breast cancer. There were positive correlation between MIAT and DUSP7 expression in breast cancer patients. We conclude that MIAT promotes breast cancer progression and functions as ceRNA to regulate DUSP7 expression by sponging miR-155-5p in breast cancer.

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Citations

Nov 4, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xinli HuangSen Lu
Jan 8, 2019·Clinical Genetics·Carolina MathiasJaqueline C de Oliveira
Jul 13, 2019·Journal of Cellular Biochemistry·Min XueXiwen Gao
Jun 6, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Waisheng ZhongJie Chen
Sep 8, 2020·Journal of Zhejiang University. Science. B·Juan XuXian-Feng Ding
Apr 11, 2020·Cancer Management and Research·Weiping YangLijun Ren
Nov 18, 2018·Journal of Cellular Physiology·Hongzhen Qin, Wenfei Liu
Jan 24, 2018·World Journal of Gastroenterology : WJG·Wen-Hua XueJie Zhao
Dec 12, 2018·Journal of Cellular Physiology·Rasoul AbdollahzadehJavad Tavakkoly-Bazzaz
Apr 18, 2019·Journal of Cellular Biochemistry·Jie WuYijian Zhang
Apr 24, 2020·Cancer Immunology Research·Shouping XuDa Pang
May 18, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ting-Fu LiShi-Jie Fu
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri

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Methods Mentioned

BETA
transfection
xenograft
PCR
flow cytometry

Software Mentioned

SPSS
MIAT

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