Long non-coding RNA MNX1-AS1 promotes migration and invasion of esophageal squamous cell carcinoma by upregulating IGF2.

European Review for Medical and Pharmacological Sciences
D-N ZhengG-P Sun

Abstract

Esophageal squamous cell carcinoma (ESCC) is a common malignant tumor with a high mortality rate and poor prognosis. In this research, we investigated the exact role of long non-coding ribonucleic acids (lncRNA) MNX1-AS1 in the metastasis of ESCC and its possible mechanism. To investigate the functions of MNX1-AS1 in ESCC, quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was utilized to detect MNX1-AS1 expression of ESCC tissues and cells. Besides, functional assays, including transwell assay and wound healing assay, were performed. Furthermore, qRT-PCR and Western blot assay were used to explore the possible underlying regulatory mechanism. The expression level of MNX1-AS1 was significantly increased in both ESCC tissues sample and cells. Moreover, knockdown of MNX1-AS1 markedly inhibited the migration and invasion of ESCC cells. Besides, knockdown of MNX1-AS1 remarkably down-regulated the mRNA and protein levels of insulin-like growth factor 2 (IGF2). Furthermore, IGF2 expression was positively correlated with MNX1-AS1 expression in ESCC tissues. MNX1-AS1 serves as a potential oncogene in ESCC, which can significantly promote ESCC cell migration and invasion by up-regulating IGF2. Our findings may provide a new ther...Continue Reading

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.