Long Non-coding RNA MRUL Contributes to Osteosarcoma Progression Through the miR-125a-5p/FUT4 Axis

Frontiers in Genetics
Cong TianShuchen Lin

Abstract

Osteosarcoma (OS) originates in the skeletal system and has a rising global incidence. Long Non-coding RNAs (lncRNAs) are key regulators of human cancers development and progression. However, their roles in the development of OS are not well understood. This research aimed to investigate the effect of a long non-coding RNA (lncRNA), MRUL, on OS and revealed its potential molecular mechanisms. The bioinformatics analysis demonstrated that lncRNA MRUL was involved in regulating nucleic acid-templated transcription, cellular macromolecule biosynthetic process, immune response, and inflammatory response. In this work, the expression of lncRNA MRUL was detected by quantitative real-time polymerase chain reaction (qRT-RCR) in both cancer tissues and cell lines. We found that lncRNA MRUL was up-regulated in cancer tissues and cell lines. Functional experiments showed that knockdown of lncRNA MRUL inhibited OS cell proliferation, and metastasis. At the same time, we found that lncRNA MRUL interacted with miR-125a-5p to suppress FUT4 expression. Moreover, inhibition of miR-125a-5p abrogated the biological roles of lncRNA MRUL knockdown on OS cell proliferation, migration, and invasion. In conclusion, these results demonstrated that OS-u...Continue Reading

References

Mar 6, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Richard Gorlick, Chand Khanna
Mar 10, 2010·Cancer Treatment and Research·Giulia Ottaviani, Norman Jaffe
Jun 2, 2011·Molecular Cancer Research : MCR·Xiaohua LiFan Daiming
Jul 23, 2011·Experimental Lung Research·Lili JiangXueshan Qiu
Jul 31, 2012·Molecular Cell·Je-Hyun YoonMyriam Gorospe
Oct 20, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jeong Kyu KimSuk Woo Nam
May 8, 2013·Journal of Nanoscience and Nanotechnology·Cornelia BraicuIoana Berindan-Neagoe
Aug 28, 2014·Biochimica Et Biophysica Acta·Guodong YangLijun Yuan
Oct 10, 2014·Journal of Cellular and Molecular Medicine·Jing ChenLong-Bang Chen
Dec 11, 2014·Journal of Hematology & Oncology·Gregory LoewenBin Shan
Mar 11, 2015·Cancer Letters·Dong HanXu Gao
Oct 16, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhaojian GongGuiyuan Li
Jun 21, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lou ZhongZhong Chen
Jul 28, 2018·The International Journal of Biochemistry & Cell Biology·Chuanyou YiYan Huang
Aug 23, 2019·Genome Research·Muller FabbriGeorge A Calin

❮ Previous
Next ❯

Citations

Oct 13, 2020·Journal of Cellular Physiology·Shenglong Li, Xiaohong Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
Transfection
Assay
PCR

Software Mentioned

Cytoscape
DAVID

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.