Long non-coding RNA NEAT1 promotes hepatocellular carcinoma cell proliferation through the regulation of miR-129-5p-VCP-IκB

American Journal of Physiology. Gastrointestinal and Liver Physiology
Luo FangPing Huang

Abstract

Long non-coding RNA nuclear-enriched abundant transcript 1 (NEAT1) plays an important role in the pathogenesis and development of several types of cancer. However, the functional mechanism of NEAT1 in hepatocellular carcinoma (HCC) remains unclear. NEAT1 and microRNA (miR)-129-5p expression in HCC tissues and cell lines was quantified by means of quantitative PCR. The effects of NEAT1 expression inhibition or upregulation in HCC cell lines were analyzed in terms of cell viability and apoptosis. Biological software was used to predict the binding sites of NEAT1 and miR-129-5p. The expression of the miR-129-5p target molecules valosin-containing protein (VCP) and IκB was detected using Western blotting. The effect of NEAT1 on tumor growth was observed in mouse models of transplanted hepatoma. In the present study, it was concluded that the expression of NEAT1 was significantly increased in the HCC tissues and cell lines. Meanwhile, after downregulating NEAT1 expression in HepG2/Huh7 cell lines, the cell viability was significantly lowered, whereas the corresponding rate of apoptosis was significantly increased. Additionally, it was found that the NEAT1 and miR-129-5p expression showed a negative correlation in HCC tissues. It was...Continue Reading

References

Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Jul 3, 2009·Genes & Development·Jeremy E WiluszDavid L Spector
Mar 11, 2010·Journal of Clinical Gastroenterology·Peter FerenciUNKNOWN World Gastroenterology Organization
Jan 5, 2011·Cancer Research·Miao-Chih TsaiHoward Y Chang
Nov 22, 2014·Nature Communications·Dimple ChakravartyMark A Rubin
Apr 10, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Lin-Jiang PanGang Chen
Aug 6, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Li ZhenYao Yi-Long
Nov 20, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Chengbiao HeQiaoyu Li
Jan 30, 2016·Journal of Experimental & Clinical Cancer Research : CR·Peng WangLingmei Qu
Mar 29, 2016·Nature Structural & Molecular Biology·Eric A Alcid, Toshio Tsukiyama
Apr 21, 2016·Journal of Cancer Research and Clinical Oncology·Jing-Wei FuXu Sun
May 6, 2016·Gene Regulation and Systems Biology·Hao KeBaowei Jiao

❮ Previous
Next ❯

Citations

Mar 3, 2018·International Journal of Molecular Sciences·Manuela LanzafameSalvatore Piscuoglio
Jun 14, 2018·Cell Cycle·Stephano Spano Mello, Laura Donatella Attardi
Nov 16, 2018·Molecular Oncology·Christiane KlecMartin Pichler
Apr 2, 2019·Journal of Cellular Physiology·Li-Yun YuanHui Xing
Feb 13, 2018·World Journal of Gastroenterology : WJG·Aya El KhodiryHend M El Tayebi
May 26, 2018·Journal of Cellular Physiology·Jianfei TuJiansong Ji
Oct 31, 2018·Frontiers in Genetics·Peixin DongHidemichi Watari
Mar 15, 2020·International Journal of Molecular Sciences·Shigemi KoyamaGoshi Shiota
Jul 20, 2019·International Journal of Oncology·Yamei PangGanghua Yang
Feb 6, 2019·International Journal of Molecular Sciences·Felix PrinzChristiane Klec
Aug 4, 2020·Liver International : Official Journal of the International Association for the Study of the Liver·Fang-Tian BuJun Li
Nov 25, 2020·Journal of Translational Medicine·Junfeng YeDi Sun
Dec 10, 2020·JHEP Reports : Innovation in Hepatology·Amin Mahpour, Alan C Mullen
Dec 11, 2020·BioMed Research International·Mohamed H YousefAnwar Abdelnaser
Nov 5, 2020·Non-coding RNA·Gabriel Pisani, Byron Baron
May 11, 2021·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
Jul 13, 2021·Frontiers in Oncology·Xinrong LinRui Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
transfection
Assay

Software Mentioned

GraphPad Prism
StarBase

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.