Long non-coding RNA NNT-AS1 sponges miR-424/E2F1 to promote the tumorigenesis and cell cycle progression of gastric cancer

Journal of Cellular and Molecular Medicine
Beibei ChenXiao-Bing Chen

Abstract

Long non-coding RNAs (lncRNAs) have been illustrated to function as important regulators in carcinogenesis and cancer progression. However, the roles of lncRNA NNT-AS1 in gastric cancer remain unclear. In the present study, we investigate the biological role of NNT-AS1 in gastric cancer tumorigenesis. Results revealed that NNT-AS1 expression level was significantly up-regulated in GC tissue and cell lines compared with adjacent normal tissue and normal cell lines. The ectopic overexpression of NNT-AS1 indicated the poor prognosis of GC patients. In vitro experiments validated that NNT-AS1 knockdown suppressed the proliferation and invasion ability and induced the GC cell cycle progression arrest at G0/G1 phase. In vivo xenograft assay, NNT-AS1 silencing decreased the tumour growth of GC cells. Bioinformatics online program predicted that miR-424 targeted the 3'-UTR of NNT-AS1. Luciferase reporter assay, RNA-immunoprecipitation (RIP) and RNA pull-down assay validated the molecular binding within NNT-AS1 and miR-424, therefore jointly forming the RNA-induced silencing complex (RISC). Moreover, E2F1 was verified to act as the target gene of NNT-AS1/miR-424, indicating the NNT-AS1/miR-424/E2F1 axis. In conclusion, our study indicat...Continue Reading

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Citations

Jul 17, 2019·Asia-Pacific Journal of Clinical Oncology·Farbod EsfandiSoudeh Ghafouri-Fard
Aug 28, 2019·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Wei ZongRongrong Jing
Mar 26, 2019·Cancer Biomarkers : Section a of Disease Markers·Shuhua ZhangHongrong Guo
Oct 17, 2020·OncoTargets and Therapy·Shulin LiXiangwei Wang
Oct 30, 2020·Cancers·Cong Zhou, Shiwei Duan
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Jul 28, 2020·Cancer Letters·Shanshan XieTianhua Zhou

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Methods Mentioned

BETA
PCR
Assay
immunoprecipitation
RIP
flow cytometry
transfection
xenograft

Software Mentioned

SPSS19
GraphPad Prism
TargetScan

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