Long non-coding RNA NORAD exhaustion represses prostate cancer progression through inhibiting TRIP13 expression via competitively binding to miR-495-3p

Cancer Cell International
Fengling ChenShuya Wang

Abstract

Prostate cancer (PCa) is a malignant heterogeneous tumor that threatens men's health. Long non-coding RNA activated by DNA damage (NORAD) and microRNA-495-3p (miR-495-3p) have been revealed to be concerned with the tumorigenesis and progression of diverse cancers. Nevertheless, the regulatory mechanism between NORAD and miR-495-3p in PCa is unclear. The expression of NORAD, miR-495-3p, and thyroid hormone receptor interactor 13 (TRIP13) mRNA was detected with quantitative real-time polymerase chain reaction (qRT-PCR). The levels of Bcl-2, Bax, Cleaved-casp-3, TRIP13, cyclin D1, and PCNA were detected through western blot analysis. The proliferation, apoptosis, migration, and invasion of PCa cells were assessed through 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), flow cytometry, or transwell assays. The relationship between NORAD or TRIP13 and miR-495-3p was confirmed via dual-luciferase reporter, RIP, or RNA pull-down assays. NORAD and TRIP13 were upregulated while miR-495-3p was downregulated in PCa tissues and cells. Both NORAD silencing and miR-495-3p upregulation accelerated cell apoptosis and curbed cell proliferation, migration, and invasion in PCa cells. Also, NORAD silencing repressed tumor grow...Continue Reading

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Citations

Oct 11, 2020·Biochemical and Biophysical Research Communications·Shaoqiu Liu, Xiaowei Xi
Nov 1, 2020·Life Sciences·Negin SoghliDurdi Qujeq
Jul 27, 2021·Frontiers in Molecular Biosciences·Liang ZengHuang Xie
Aug 23, 2021·Hematology/oncology and Stem Cell Therapy·Mohammad Masoud EslamiMohammad Ahmadvand

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Methods Mentioned

BETA
flow cytometry
electrophoresis
immunoprecipitation
RIP
pull-down
transfection
transfecttion
xenograft

Software Mentioned

SPSS
GraphPad Prism
ImageJ
NORAD

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