Long non-coding RNA PARTICLE bridges histone and DNA methylation

Scientific Reports
Valerie Bríd O'LearyMichael John Atkinson

Abstract

PARTICLE (Gene PARTICL- 'Promoter of MAT2A-Antisense RadiaTion Induced Circulating LncRNA) expression is transiently elevated following low dose irradiation typically encountered in the workplace and from natural sources. This long non-coding RNA recruits epigenetic silencers for cis-acting repression of its neighbouring Methionine adenosyltransferase 2A gene. It now emerges that PARTICLE operates as a trans-acting mediator of DNA and histone lysine methylation. Chromatin immunoprecipitation sequencing (ChIP-seq) and immunological evidence established elevated PARTICLE expression linked to increased histone 3 lysine 27 trimethylation. Live-imaging of dbroccoli-PARTICLE revealing its dynamic association with DNA methyltransferase 1 was confirmed by flow cytometry, immunoprecipitation and direct competitive binding interaction through electrophoretic mobility shift assay. Acting as a regulatory docking platform, the long non-coding RNA PARTICLE serves to interlink epigenetic modification machineries and represents a compelling innovative component necessary for gene silencing on a global scale.

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Citations

Nov 23, 2017·Nucleic Acids Research·Diogo M RibeiroChristine Brun
Sep 1, 2017·The Journal of Cell Biology·Ronald L NepplKenneth Walsh
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May 7, 2019·Journal of Cellular Physiology·Valerie B O'LearyMichael J Atkinson
Nov 19, 2019·Critical Reviews in Clinical Laboratory Sciences·Yan ZhangYahong Li
Jul 31, 2020·Journal of Cellular Physiology·Xiaolong XuJingwei Zhang
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Apr 27, 2021·Frontiers in Molecular Biosciences·Nikita Fernandes, J Ross Buchan
Jul 3, 2021·Brain Sciences·Markéta PokornáValerie Bríd O'Leary

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Methods Mentioned

BETA
ELISA
transfection
ChIP-seq
Flow cytometry
electrophoretic mobility shift assay
immunoprecipitation
electrophoresis
ChIP
PCR

Software Mentioned

PARTICLEs
Integrative Genomics Viewer
INGENUITY
FIGI
PARTICLE
INGENUITY pathway analysis ( IPA )
Triplex Domain Finder (

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