Long non-coding RNA PVT1 promotes autophagy as ceRNA to target ATG3 by sponging microRNA-365 in hepatocellular carcinoma

Gene
Liang YangJingang Liu

Abstract

The pathogenesis and the underlying mechanisms of hepatocellular carcinoma (HCC) remain unclear. LncRNA plasmacytoma variant translocation 1 (PVT1) is an oncogene in a variety of cancers. The role of PVT1 in HCC progression is not completely understood. In the present study, we conducted a series of studies to explore the role of PVT1 on autophagy in HCC cells. Our study found PVT1 levels were markedly up-regulated in the corresponding HCC tissues. Importantly, we found that PVT1 could facilitate cell autophagy in HCC cells. Then, we confirmed that the effect of PVT1 promoting autophagy was dependent on regulating ATG3 expression. Further investigations revealed that PVT1 could upregulate autophagy-related gene 3 (ATG3) expression by acting as an endogenous sponge of miR-365, which was an inhibitor gene on ATG3 protein by targeting 3'UTR of ATG3 mRNA. Moreover, rescue assays indicated that the effect of PVT1 on autophagy of HCC cells were dependent on miR-365. In conclusion, our study demonstrated PVT1 might be a key regulator participating in autophagy in HCC cells. We proved that PVT1 could promote autophagy as ceRNA by targeting miR-365.

Citations

Jul 13, 2019·Journal of Cellular Physiology·Soudeh Ghafouri-FardMohammad Taheri
Nov 30, 2019·Frontiers in Oncology·Ariadna BoloixMiguel F Segura
Aug 28, 2020·World Journal of Gastroenterology : WJG·Zhao-Shan NiuLi-Mei-Li Tian
Aug 6, 2019·Frontiers in Oncology·Wenxi WangZhaoyang Zeng
Jun 30, 2019·Frontiers in Oncology·Camille DerderianOlorunseun O Ogunwobi
Jan 28, 2021·International Journal of Molecular Sciences·Claire Wilson, Aditi Kanhere
Feb 3, 2021·Autophagy·Soudeh Ghafouri-FardMohammad Taheri
Jul 11, 2019·Journal of Molecular Cell Biology·Ya-Jie XuChang-Mei Liu
Oct 30, 2020·Pharmacogenomics and Personalized Medicine·Chenyu YangChengzhan Zhu
Feb 10, 2021·Cell Death Discovery·Na JiangLei Shang
May 11, 2021·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
Jul 9, 2021·Frontiers in Cell and Developmental Biology·Dongmei FangMin Li
Aug 22, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mohammad Hossein PourhanifehHamed Mirzaei
Dec 4, 2021·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·H L LiH Y Yang

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