Long non-coding RNA PVT1 promotes malignancy in human endometrial carcinoma cells through negative regulation of miR-195-5p

Biochimica Et Biophysica Acta. Molecular Cell Research
Fanfei KongXiaoxin Ma

Abstract

The plasmacytoma variant translocation 1 (PVT1)1 gene is a long non-coding RNA (lncRNA)2 that has been shown to be an oncogene in many cancers. Herein, the function and potential molecular mechanisms connecting PVT1 and miR-195-5p were elucidated in endometrial cancer cell lines. Quantitative real-time PCR and fluorescence in situ hybridization (FISH)3 demonstrated that PVT1 is up-regulated concomitant with miR-195-5p down-regulation in human endometrial carcinoma tissues. PVT1 knockdown inhibited cell proliferation, migration, and invasion while facilitating apoptosis of endometrial cancer cells. Moreover, restoration of miR-195-5p due to PVT1 knockdown exerted tumor-suppressive functions. We observed that PVT1 promotes malignant cell behavior by decreasing miR-195-5p expression. Binding of PVT1 and miR-195-5p was confirmed using luciferase assays. Furthermore, expression of miR-195-5p negatively correlates with PVT1 expression. At the molecular level, either PVT1 knockdown or miR-195-5p overexpression resulted in a decrease of acidic fibroblast growth factor receptor (FGFR1)4 and basic fibroblast growth factor (FGF2).5 FGFR1 and FGF2 are targets of miR-195-5p that play a critical role in endometrial carcinoma by activating PI...Continue Reading

Citations

Mar 26, 2019·Cancer Biomarkers : Section a of Disease Markers·Jiyang Fang, Jianyue Huang
Jul 13, 2019·Journal of Cellular Physiology·Soudeh Ghafouri-FardMohammad Taheri
Nov 27, 2019·Technology in Cancer Research & Treatment·Xinquan WuKuirong Jiang
Jun 27, 2019·Journal of Cellular Physiology·Peijie DuGuijun Qin
Feb 23, 2020·Frontiers in Oncology·Águeda Martínez-BarriocanalHiginio Dopeso
Nov 17, 2020·Frontiers in Oncology·Olorunseun O Ogunwobi, Miguel F Segura
Apr 4, 2021·International Journal of Molecular Sciences·Roberto PiergentiliFederica Perelli
Aug 28, 2021·Healthcare·Anna Franca CavaliereFabrizio Signore

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