Long non-coding RNA RP11-552M11.4 promotes cells proliferation, migration and invasion by targeting BRCA2 in ovarian cancer

Cancer Science
Kejin HuangJing Wang

Abstract

The present study aimed to investigate the effect of long non-coding RNA (lncRNA) RP11-552M11.4 on cell proliferation, apoptosis, migration and invasion as well as its targeting genes in epithelial ovarian cancer (EOC) cells. LncRNA RP11-552M11.4 expression was detected in 67 tumor tissues and paired adjacent tissues obtained from EOC patients. lncRNA RP11-552M11.4 mimic/inhibitor plasmids were transferred into ovarian cancer cells (SKOV3, A-2780) and normal ovarian epithelial cells (IOSE80 cells). In addition, rescue experiment was carried out by transferring BRCA2 inhibitor&lncRNA RP11-552M11.4 inhibitor plasmids into SKOV3 and A-2780 cells. qPCR, western blot, CKK-8, Annexin V/propidium iodide (AV/PI), wound-healing and Matrigel invasion assays were carried out to detect RNA expression, protein expression, cell proliferation, apoptosis, migration, and invasion, respectively. LncRNA RP11-552M11.4 expression was elevated in tumor tissues compared with paired adjacent tissues and correlated with higher pathological grade, International Federation of Gynecology and Obstetrics stage and worse overall survival in EOC patients. LncRNA RP11-552M11.4 promoted SKOV3 cell proliferation, migration and invasion whereas it inhibited apopt...Continue Reading

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Citations

Nov 30, 2018·Journal of Cellular Physiology·Jianfei TuJiansong Ji
Jun 20, 2020·The Kaohsiung Journal of Medical Sciences·Lai-Gang ZhaoQin-Fen Li
Nov 27, 2020·International Journal of Molecular Sciences·Eleonora A BragaNikolay E Kushlinskii
Feb 24, 2021·Pathology, Research and Practice·Elahe Seyed HosseiniHossein Nikzad
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Jun 23, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Alexey V ZamaraevBoris Zhivotovsky
May 15, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lingling TongXiaochun Sun

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Datasets Mentioned

BETA
AB073614

Methods Mentioned

BETA
transfection
X‐ray
scraping
flow cytometry

Software Mentioned

GraphPad Prism
Image J
LncTar
Arraystar
SPSS
RIsearch
RNAplex
DisGeNET
Arraystar Human LncRNA

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