Long non-coding RNA SLCO4A1-AS1 drives the progression of non-small-cell lung cancer by modulating miR-223-3p/IKKα/NF-κB signaling.

Cancer Biology & Therapy
Qingpeng LiHaitao Ma

Abstract

Globally, lung cancer is known as a major cause of cancer-associated death and non-small-cell lung cancer (NSCLC) accounts for majority of all cases. Growing evidence has emerged that long non-coding RNAs (lncRNAs) act as vital regulatory molecules in various malignancies. Nevertheless, the function of SLCO4A1 antisense RNA 1(SLCO4A1-AS1) in NSCLC is vague. This study intended to investigate the biological role and probable regulatory mechanism of SLCO4A1-AS1 in NSCLC. qRT-PCR revealed that SLCO4A1-AS1 level was upregulated in NSCLC. Function assays manifested that silence of SLCO4A1-AS1 attenuated NSCLC cell proliferation, migration and invasion but promoted NSCLC cell apoptosis. Furthermore, we disclosed that SLCO4A1-AS1 activated NF-κB pathway in NSCLC, and that IKKα, an NF-κB pathway-related gene, possessed an enhanced level in NSCLC tissues and cells. Importantly, miR-223-3p bound with SLCO4A1-AS1 and IKKα. Further, SLCO4A1-AS1 competitively bound with miR-223-3p to increase IKKα expression, thereby activating NF-κB signaling pathway. In conclusion, SLCO4A1-AS1 drove NSCLC progression by activating NF-κB signaling pathway via sponging miR-223-3p to enhance IKKα expression. Thus, SLCO4A1-AS1 might be a promising biomarker f...Continue Reading

References

Jun 7, 2000·Lancet·P C HoffmanE E Vokes
Dec 22, 2009·Molecular BioSystems·Benjamin Z Stanton, Lee F Peng
Jul 29, 2015·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Noori Akhtar-Danesh, Christian Finley
Nov 6, 2015·BioMed Research International·Sudheendra RaoDamien D Pearse
Jan 25, 2018·Nature·Roy S HerbstChris Boshoff
Apr 28, 2018·Biochemical and Biophysical Research Communications·De-Gang JiHong-Yu Liu
Sep 12, 2018·Journal of Experimental & Clinical Cancer Research : CR·Jiangtao YuChunfang Song
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lei ZhangKangsheng Tu
Dec 16, 2018·Biochemical and Biophysical Research Communications·Weifeng SunShijie Xin
Oct 9, 2019·Aging and Disease·Brian Z WangKunlin Jin

❮ Previous
Next ❯

Citations

Mar 24, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
immunoprecipitation
flow cytometry
nuclear translocation
RIP
transfection

Software Mentioned

DIANA
SPSS

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis