Long non-coding RNA SNHG1 predicts a poor prognosis and promotes colon cancer tumorigenesis

Oncology Reports
Huan YangZheng Jiang

Abstract

Colon cancer is the main cause of cancer mortality worldwide. Its poor prognosis is mainly ascribed to high recurrence rates. Identifying novel prognostic biomarkers and therapeutic key points for management is crucial and important. Long non-coding RNAs (lncRNAs) are a class of RNAs, which have various roles in carcinogenicity and molecular mechanisms. The lncRNA small nucleolar RNA host gene 1 (SNHG1) contributes to the promotion of tumor development, however, the connections between SNHG1 and colon cancer are still unclear. The aim of the present study was to investigate the clinical significance, the biological functions, and the potential mechanism of SNHG1 in colon cancer. In the present study, we referred to the Oncomine database and used RT-qPCR to determine that SNHG1 expression was significantly higher both in colon cancer tissues and cancerous cell lines than in normal samples. Cell functional experiments were performed after knockdown of SNHG1, including Cell Counting Kit-8 assay, colony formation assay, Transwell® assay, and flow cytometric analyses of cell apoptosis, which suggested that SNHG1 stimulated colon cancer cell proliferation, promoted cell invasion and migration, and inhibited apoptosis. Immunohistochem...Continue Reading

Citations

Apr 23, 2020·Frontiers in Oncology·Alina-Andreea ZimtaIoana Berindan-Neagoe
Apr 26, 2020·Journal of Clinical Medicine·Dino BekricChristian Mayr
May 1, 2021·International Journal of Molecular Sciences·Francesca BaldiniAldo Pagano

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Datasets Mentioned

BETA
GSE31737

Methods Mentioned

BETA
PCR
Reverse
fluorescence
transfection
flow cytometry
protein assay
electrophoresis
chip
ubiquitination

Software Mentioned

SPSS
GraphPad Prism
ImageJ
Oncomine
GraphPad

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