Long non-coding RNA XIST contributes to osteoarthritis progression via miR-149-5p/DNMT3A axis.

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Yunke LiuJia Zheng

Abstract

Long non-coding RNAs (lncRNAs) are largely involved in the development of osteoarthritis (OA), a chronic and degenerative joint disease. The objective of this paper is to research the functional role and molecular mechanism of lncRNA X inactive specific transcript (XIST) in OA. The levels of XIST, microRNA-149-5p (miR-149-5p), and DNA methyltransferase 3A (DNMT3A) were measured. Cell viability and apoptosis rate were determined. Associated protein levels were examined through Western blot. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were implemented for confirming the target relation. And the role of XIST on OA in vivo was investigated by a rat model. XIST was expressed at a high level in OA cartilage tissues and IL-1β-treated chondrocytes. XIST knockdown promoted cell viability but restrained cell apoptosis and extracellular matrix (ECM) protein degradation in IL-1β-treated chondrocytes. XIST directly targeted miR-149-5p and miR-149-5p down-regulation restored si-XIST-mediated pro-proliferative and anti-apoptotic or ECM degradative effects. DNMT3A was a target gene of miR-149-5p and DNMT3A overexpression ameliorated miR-149-5p-induced promotion of cell viability but repression of apoptosis and ECM de...Continue Reading

Citations

Jan 26, 2021·The Journal of Gene Medicine·Qiwei FuLexiang Li
Jun 22, 2021·International Immunopharmacology·Shengqiang FuDun Liu
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Wenlun WangLingyun Zhu
Aug 8, 2021·International Journal of Molecular Sciences·Chenshuang Li, Zhong Zheng
Sep 10, 2021·Cell Proliferation·Hesuyuan HuangJianhao Lin
Nov 23, 2021·Frontiers in Endocrinology·Shabana Amanda AliIgor Jurisica

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