Long non-coding RNAs control hematopoietic stem cell function

Cell Stem Cell
Min LuoMargaret A Goodell

Abstract

Hematopoietic stem cells (HSCs) possess unique gene expression programs that enforce their identity and regulate lineage commitment. Long non-coding RNAs (lncRNAs) have emerged as important regulators of gene expression and cell fate decisions, although their functions in HSCs are unclear. Here we profiled the transcriptome of purified HSCs by deep sequencing and identified 323 unannotated lncRNAs. Comparing their expression in differentiated lineages revealed 159 lncRNAs enriched in HSCs, some of which are likely HSC specific (LncHSCs). These lncRNA genes share epigenetic features with protein-coding genes, including regulated expression via DNA methylation, and knocking down two LncHSCs revealed distinct effects on HSC self-renewal and lineage commitment. We mapped the genomic binding sites of one of these candidates and found enrichment for key hematopoietic transcription factor binding sites, especially E2A. Together, these results demonstrate that lncRNAs play important roles in regulating HSCs, providing an additional layer to the genetic circuitry controlling HSC function.

References

Apr 1, 1996·The Journal of Experimental Medicine·M A GoodellR C Mulligan
Oct 1, 2003·Nature Immunology·Valérie PouillonStéphane Schurmans
Mar 29, 2008·Cell Stem Cell·Stuart M ChambersMargaret A Goodell
Feb 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Craig L SemeradCornelis Murre
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ryan M O'ConnellDavid Baltimore
Nov 12, 2010·The New England Journal of Medicine·Timothy J LeyRichard K Wilson
Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Matthew D SimonRobert E Kingston
Jul 28, 2012·Genome Research·Michael R TallackAndrew C Perkins
Aug 22, 2012·Annual Review of Genetics·Gioacchino Natoli, Jean-Christophe Andrau
Nov 13, 2012·Cell Stem Cell·Eric R LechmanLuigi Naldini
Nov 28, 2012·Genome Biology·David Kelley, John Rinn
Jan 29, 2013·Cell·Carla A KlattenhoffLaurie A Boyer
Feb 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Alla A SigovaRichard A Young
Feb 19, 2013·Cell·Eda YildirimJeannie T Lee
May 23, 2013·Blood·Margaret A Goodell, Lucy A Godley

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Citations

Feb 18, 2016·Stem Cells International·Shanshan Hu, Ge Shan
Dec 15, 2015·Annals of the New York Academy of Sciences·Aditi VediElisa Laurenti
Mar 10, 2016·Briefings in Functional Genomics·Marion A SalvadorChristophe Ginestier
May 21, 2015·Immunity·Ansuman T Satpathy, Howard Y Chang
May 26, 2016·Genomics, Proteomics & Bioinformatics·Tangliang LiZhao-Qi Wang
Apr 27, 2017·International Immunology·Wooseok Seo, Ichiro Taniuchi
Jun 21, 2017·The Journal of Cell Biology·Gregoire StikCharles Durand
Jul 4, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Bing Zhang, Ya-Chieh Hsu
Dec 22, 2017·Journal of Cellular Biochemistry·Ayman El-Sayed ShafeiAmal S El-Shal
Sep 21, 2017·Blood·Juan R Alvarez-Dominguez, Harvey F Lodish
May 23, 2018·Database : the Journal of Biological Databases and Curation·Fulong YuShujun Cheng
Sep 7, 2017·ELife·M Joaquina DelásGregory J Hannon
Aug 11, 2016·F1000Research·Xu Zhang, Wenqian Hu
Nov 21, 2018·Aging Cell·António Sousa-FrancoBruno Bernardes de Jesus
Mar 1, 2019·Journal of Leukocyte Biology·Megha BasavappaJorge Henao-Mejia
Aug 23, 2017·Nature Immunology·Y Grace ChenHoward Y Chang
Apr 24, 2019·Non-coding RNA·Marina R Hadjicharalambous, Mark A Lindsay
Jul 25, 2019·Frontiers in Oncology·Michelle NgJan-Henning Klusmann
Jan 4, 2018·Blood Advances·Chi-Yuan YaoHwei-Fang Tien
Jan 25, 2018·Blood Advances·Wayne A WarnerDaniel C Link
Jan 10, 2018·Oncotarget·Noam FallikRoi Gazit
Aug 16, 2016·Nature·Jonathan J KotzinJorge Henao-Mejia
Aug 11, 2017·Nature Communications·Adrian SchwarzerJan-Henning Klusmann
Jun 7, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Haiyun QianGuopei Zheng
Aug 21, 2018·Nucleic Acids Research·Benoit P NicoletMonika C Wolkers
Nov 15, 2019·Nature Communications·Jordan P LewandowskiJohn L Rinn
Apr 8, 2020·Journal of Hematology & Oncology·Xia LiHe Huang
Feb 1, 2017·Molecular Cancer·Xuefei WangJi-Long Chen
Jul 28, 2017·Open Biology·M Joaquina Delás, Gregory J Hannon
Jul 6, 2020·Annals of Hematology·Leonidas BenetatosGeorgios Vartholomatos
Apr 17, 2018·Non-coding RNA·Amanda Salviano-SilvaMaria Luiza Petzl-Erler
Jun 12, 2020·Molecular Oncology·Pedro BlecuaManel Esteller
Oct 27, 2018·Current Stem Cell Reports·Nathaniel Magilnick, Mark P Boldin

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