Long noncoding RNA AOC4P regulates tumor cell proliferation and invasion by epithelial-mesenchymal transition in gastric cancer

Therapeutic Advances in Gastroenterology
Kecheng ZhangLin Chen

Abstract

The clinical relevance and biological role of tissular AOC4P in gastric cancer (GC) remains to be clarified. The association between AOC4P expression and clinicopathological characteristics was investigated. In vitro, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, wound healing and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed to explore the biological effects of AOC4P on GC cell proliferation, migration, invasion, and apoptosis in MGC-803 and BGC-823 cell lines. In vivo, animal experiments were conducted to confirm the in vitro findings. Quantitative real-time polymerase chain reaction, western blotting, and immunofluorescence were used to investigate the potential mechanisms. Expression levels of AOC4P were significantly higher in tumor tissues than in noncancerous tissues, and patients with high levels of AOC4P had poor overall and disease-free survival. AOC4P expression was correlated with lymphovascular invasion. In vitro, knockdown of AOC4P inhibited tumor cell proliferation, migration, and invasion, and promoted apoptosis of MGC-803 and BGC-823 cells. In vivo, BGC-823 cells transfected with AOC4P siRNA formed smaller and lighter tumors th...Continue Reading

References

Sep 8, 2012·Nature·Sarah DjebaliThomas R Gingeras
Aug 1, 2014·Nature·UNKNOWN Cancer Genome Atlas Research Network
Sep 23, 2014·International Journal of Cancer. Journal International Du Cancer·Hongmei ZengXue Qin Yu
Dec 3, 2014·Cancer Letters·Xin-yu FangDong-qing Ye
Jun 10, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ling LiFang Zhou
Oct 7, 2015·Trends in Cell Biology·Xin Ye, Robert A Weinberg
Jan 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·Kenzui TaniueTetsu Akiyama
Dec 14, 2016·Cancer Cell·Annette BeckerDavid Lyden
Jan 13, 2017·Seminars in Cell & Developmental Biology·Richard J LobbAndreas Möller
Mar 30, 2017·World Journal of Gastroenterology : WJG·Hong-Zhi ShiLin Chen

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Methods Mentioned

BETA
electrophoresis
Fluorescence imaging
xenografts
transfection
ubiquitination

Software Mentioned

- Quest
Tissue
SPSS

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