Long noncoding RNA DANCR, working as a competitive endogenous RNA, promotes ROCK1-mediated proliferation and metastasis via decoying of miR-335-5p and miR-1972 in osteosarcoma.

Molecular Cancer
Yong WangZhi Lu

Abstract

Accumulating evidences indicate that non-coding RNAs (ncRNAs) including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) acting as crucial regulators in osteosarcoma (OS). Previously, we reported that Rho associated coiled-coil containing protein kinase 1 (ROCK1), a metastatic-related gene was negatively regulated by microRNA-335-5p (miR-335-5p) and work as an oncogene in osteosarcoma. Whether any long non-coding RNAs participate in the upstream of miR-335-5p/ROCK1 axial remains unclear. Expression of differentiation antagonizing non-protein coding RNA (DANCR) and miR-335-5p/miR-1972 in osteosarcoma tissues were determined by a qRT-PCR assay and an ISH assay. Osteosarcoma cells' proliferation and migration/invasion ability changes were measured by a CCK-8/EDU assay and a transwell assay respectively. ROCK1 expression changes were checked by a qRT-PCR assay and a western blot assay. Targeted binding effects between miR-335-5p/miR-1972 and ROCK1 or DANCR were verified by a dual luciferase reporter assay and a RIP assay. In vivo experiments including a nude formation assay as well as a CT scan were applied to detect tumor growth and metastasis changes in animal level. In the present study, an elevated DNACR was found in osteo...Continue Reading

References

Nov 30, 2005·Nucleic Acids Research·Caifu ChenKarl J Guegler
Mar 10, 2010·Cancer Treatment and Research·Giulia Ottaviani, Norman Jaffe
Aug 2, 2011·Cell·Leonardo SalmenaPier Paolo Pandolfi
Aug 27, 2013·Molecular and Cellular Biochemistry·Yong WangQin Fu
Jun 14, 2014·Advances in Experimental Medicine and Biology·Lisa A Kafchinski, Kevin B Jones
Aug 15, 2014·BoneKEy Reports·Brian Ell, Yibin Kang
Aug 28, 2014·Biochimica Et Biophysica Acta·Guodong YangLijun Yuan
May 13, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sheng-xian YuanWei-ping Zhou
Sep 18, 2015·Frontiers in Pediatrics·Valerie B SampsonE Anders Kolb
Apr 6, 2016·Current Genomics·Chi Han Li, Yangchao Chen
Sep 4, 2017·Journal of Cellular and Molecular Medicine·Lijun CaiHuilin Yang
Nov 14, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Zhigang QinQiaoling Zhou
Dec 27, 2017·Biochemical and Biophysical Research Communications·Hongliang LiShangyong Zheng
Dec 27, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jinguan LinZhe He

❮ Previous
Next ❯

Citations

Jan 22, 2020·The International Journal of Biological Markers·Feifei LiuJun Wang
Jun 3, 2020·The Journal of International Medical Research·Hong ChenMan Zhang
Sep 18, 2020·Biomarkers in Medicine·Cheng-Ze ChenJin-Miao Qu
Nov 14, 2018·Journal of Experimental & Clinical Cancer Research : CR·Yonghao ZhanLiqun Zhou
Oct 27, 2018·Journal of Cellular Physiology·Huiling LiangYuanbo Cui
Jan 18, 2019·Experimental and Therapeutic Medicine·Fukun ChenZhiyong Deng
Apr 12, 2019·Journal of Experimental & Clinical Cancer Research : CR·Rilei JiangXu Zhang
Feb 23, 2019·International Journal of Immunopathology and Pharmacology·Jun ChenYong Zhang
Jul 6, 2019·Journal of Cellular and Molecular Medicine·Hongjuan SongGuilin Li
Sep 11, 2020·BioMed Research International·Hua GaoZuqiang Yi
Jan 29, 2019·Frontiers in Pharmacology·Qiang LuFenghua Zhou
Feb 19, 2019·Cancer Cell International·Ming-Hui MaDong-Qiu Dai
Dec 5, 2019·Frontiers in Oncology·Shi-Jia JinWei-Lin Jin
Aug 14, 2020·Cancer Biotherapy & Radiopharmaceuticals·Rui Ou, Xin Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
immunoprecipitation assay
X-ray
transfection
Assay
immunoprecipitation
RIP
xenograft

Software Mentioned

LncBase
DANCR
Image
GraphPad Prism
Pro Plus
DIANA
SPSS
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.