Long noncoding RNA DIO3OS interacts with miR-122 to promote proliferation and invasion of pancreatic cancer cells through upregulating ALDOA

Cancer Cell International
Kang CuiWang Ma

Abstract

Long noncoding RNA (lncRNA) has been implicated in numerous tumors, including pancreatic cancer (PC). However, the precise cellular roles and molecular mechanisms of lncRNA DIO3OS on PC development remains to be fully clarified. We performed the meta-analysis on PC samples and non-tumor samples retrieved from the TCGA database, and measured the levels of DIO3OS in PC cell lines and a normal pancreatic duct epithelial cell line HPDE6-C7. Cell proliferation was evaluated via CCK-8 assay. Cell invasion in vitro was investigated by transwell assay. The RNA immunoprecipitation assay and luciferase reporter assay was utilized to confirm the putative miR-122-binding site in DIO3OS. The effects of DIO3OS on PC progression were tested using in vivo subcutaneous xenografts. Our results showed that DIO3OS was highly expressed in human PC tissues and PC cell lines. DIO3OS exhibited oncogenic properties in stimulating PC cell proliferation and invasion in vitro and promoting cancer growth in vivo. Through online predictive tools and functional experiments, we found that DIO3OS could bind directly to microRNA-122 (miR-122) and inhibited its expression, which functioned as a tumor suppressor in PC cells. We also verified that ALDOA was the di...Continue Reading

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Citations

Dec 2, 2020·Journal of Experimental & Clinical Cancer Research : CR·Lidong CaoRanji Cui
Oct 16, 2020·Journal of Cell Communication and Signaling·Mingming WangJiangsheng Huang
May 27, 2021·Transcription·K T Ramya DeviSanjana Prakash Hemdev

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Methods Mentioned

BETA
surgical resection
transfection
PCR
immunoprecipitation
RIP
xenografts
xenograft

Software Mentioned

UALCAN
SPSS
TargetScan

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