Long noncoding RNA expression profile and association with SLEDAI score in monocyte-derived dendritic cells from patients with systematic lupus erythematosus

Arthritis Research & Therapy
Yilun WangJinhua Xu

Abstract

Monocyte-derived dendritic cells (moDCs) play important roles in the pathogenesis of systemic lupus erythematosus (SLE). Aberrant expression of long noncoding RNAs (lncRNAs) could affect the function of moDCs. The aim of this study was to explore the lncRNA expression profile in moDCs of SLE patients to provide new insights into SLE. LncRNA and mRNA microarrays were performed to identify differentially expressed lncRNAs and mRNAs in moDCs of SLE patients compared with normal controls. Bioinformatics analysis was also performed. Quantitative polymerase chain reaction (qPCR) was used to validate the results, and correlation analysis was used to analyze the relationship between these aberrantly expressed lncRNAs and SLE disease activity index (SLEDAI) scores. According to the gene expression profiles, 163 lncRNAs were differentially expressed between SLE and normal controls, including 118 that were upregulated and 45 that were downregulated. A total of 137 mRNAs were differentially expressed in moDCs of patients with SLE, including 83 that were upregulated and 54 that were downregulated. Furthermore, qPCR data showed that lncRNA ENST00000604411.1 (18.23-fold, P < 0.001) and ENST00000501122.2 (1.96-fold, P < 0.001) were upregulated...Continue Reading

References

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Citations

Jul 18, 2020·Biomolecules·Valeria LoddeMaria Laura Idda
Jul 13, 2020·Current Rheumatology Reports·Tayfun Hilmi AkbabaSeza Ozen
Jun 26, 2020·Biomolecules·Mohammad TaheriSoudeh Ghafouri-Fard
Mar 2, 2021·Molecular Diagnosis & Therapy·Alejandra ReolidE Daudén
Aug 4, 2020·Journal of Translational Autoimmunity·Yaoyao Zou, Hanshi Xu

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Datasets Mentioned

BETA
GSE89240

Methods Mentioned

BETA
flow cytometry

Software Mentioned

Cytoscape
RNAplex

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