Long noncoding RNA expression profile in HLE B-3 cells during TGF-β2-induced epithelial-mesenchymal transition

BMC Ophthalmology
Bingyu ZhangZhixiang Ding

Abstract

Recent evidence has shown that long noncoding RNAs (lncRNAs) are involved in the process of epithelial-mesenchymal transition (EMT). However, little research has focused on the expression profile of lncRNAs during EMT in human lens epithelial cells (LECs) and their functions have not yet been described. Dysregulated lncRNAs and mRNAs in normal human lens epithelial B-3(HLE B-3) cells and during transforming growth factor β2(TGF-β2)-induced EMT were analyzed via lncRNA microarray. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analyses of differentially expressed mRNAs were performed to identify their functions and pathologic pathways. Six candidate lncRNAs were validated via quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR) to confirm the microarray data. A total of 775 lncRNAs (325 up-regulated and 450 down-regulated) and 935 mRNAs (329 up-regulated and 606 down-regulated) were differentially expressed in HLE B-3 cells during TGF-β2-induced EMT compared to normal HLE B-3 cells. GO and KEGG Pathway analyses indicated the functions of differentially expressed mRNAs in the TGF-β2-induced EMT in HLE B-3 cells. qRT-PCR confirmed the trends indicated in microarray analysis...Continue Reading

References

Nov 20, 1991·JAMA : the Journal of the American Medical Association·C Marwick
Jan 1, 1996·Journal of Cataract and Refractive Surgery·S SaikaA Yamanaka
Feb 24, 2000·The British Journal of Ophthalmology·W R MeacockM R Stanford
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 17, 2002·Experimental Eye Research·I Michael Wormstone
Dec 19, 2003·Nucleic Acids Research·Minoru KanehisaMasahiro Hattori
Jan 27, 2004·The American Journal of Pathology·Shizuya SaikaAnita B Roberts
Dec 31, 2005·Nucleic Acids Research·UNKNOWN Gene Ontology Consortium
Sep 6, 2007·Genome Research·Sorin DraghiciRoberto Romero
Nov 18, 2008·Experimental Eye Research·I Michael WormstoneChristopher S C Liu
Feb 26, 2009·Cell·Chris P PontingWolf Reik
Apr 15, 2009·Archives of Ophthalmology·Niranjan AwasthiB J Wagner
Jun 3, 2009·The Journal of Clinical Investigation·Michael Zeisberg, Eric G Neilson
Jul 3, 2009·Genes & Development·Jeremy E WiluszDavid L Spector
Nov 19, 2011·Cancer Discovery·John R Prensner, Arul M Chinnaiyan
Jun 6, 2012·Annual Review of Biochemistry·John L Rinn, Howard Y Chang
Oct 24, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Thomas C RobertsMarc S Weinberg
Jun 13, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chenchao MaKaixing Ai

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Methods Mentioned

BETA
Protein Assay
Feature Extraction

Software Mentioned

Feature Extraction
SPSS
GeneSpring

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