Long Noncoding RNA GAS5 Suppresses 3T3-L1 Cells Adipogenesis Through miR-21a-5p/PTEN Signal Pathway

DNA and Cell Biology
Hai Feng LiuMing Zhou Li

Abstract

Emerging studies indicated that both long noncoding RNAs and micro-RNAs play crucial roles in the mediation of adipogenesis, which is closely linked to obesity-related diseases. However, the mechanisms of lncRNA-miRNAs coregulating in adipogenesis are still largely unknown. In this study, we determined that lncRNA growth arrest-specific 5 (GAS5) presented an opposite expression pattern with miR-21a-5p in 3T3-L1 adipocytes development. To explore the role of GAS5 in adipogenesis, pcDNA3.1-GAS5 expression vectors and GAS5-siRNAs were used to perform GAS5 overexpression and knockdown, respectively. Ectopic expression of GAS5 dramatically reduced miR-21a-5p level and suppressed the proliferation of 3T3-L1 preadipocytes, while silencing GAS5 slightly increased miR-21a-5p expression but had no significant influence on the cell viability. In addition, overexpression of GAS5 remarkably decreased the mRNA and protein levels of adipogenic marker genes, and resulted in a notable reduction of lipid accumulation. In contrast, overexpressing miR-21a-5p significantly facilitated differentiation of 3T3-L1 cells. By target gene prediction and luciferase reporter assay, we suggested that GAS5 might indirectly improve the expression of phosphatas...Continue Reading

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Citations

Apr 24, 2019·Diabetes·Lei Sun, Jiandie D Lin
Sep 29, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Songjia LaiShiyi Chen
Aug 9, 2020·Frontiers in Endocrinology·Tie-Ning ZhangChun-Feng Liu
May 18, 2021·Non-coding RNA Research·Guannan DuYi Li

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Methods Mentioned

BETA
PCR
Assay
Transfection
electrophoresis
immunoprecipitation assay
RNA-Seq

Software Mentioned

GraphPad Prism
TargetScan
RNAhybrid

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