Long noncoding RNA H19 contributes to gallbladder cancer cell proliferation by modulated miR-194-5p targeting AKT2

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Shou-Hua WangZhi-Wei Quan

Abstract

Gallbladder cancer (GBC) is a highly malignant cancer with poor prognosis. Although long noncoding RNA (lncRNA) H19 has been reported to play vital role in many human cancers, whether it is involved in GBC proliferation is still unknown. This study was designed to explore the effect of H19 in GBC cell proliferation. The expression of H19 and AKT2 were significantly elevated in GBC tissues, and the level of miR-194-5p is markedly decreased. Moreover, the RNA levels of H19 and AKT2 were positively correlated, and H19 elevation was significantly associated with tumor size. Cell proliferation decreased significantly after knockdown of H19 in GBC-SD and NOZ cells and after knockdown of AKT2 in NOZ cells. Results from cell cycle studies indicated that the S phase were significantly decreased after knockdown of H19 in NOZ cells but significantly elevated after overexpression of H19 in GBC-SD cells. Furthermore, knockdown of H19 upregulated miR-194-5p levels, yet significantly decreased miR-194-5p targeting AKT2 gene expression in NOZ cells. Inhibitor against miR-194-5p reversed these effects. In addition, overexpression of H19 in GBC-SD cells downregulated miR-194-5p and markedly increased AKT2 expression, and miR-194-5p mimic reverse...Continue Reading

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Citations

Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shou-Hua WangZhi-Wei Quan
Jan 26, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tao HuangBin Wang
May 4, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yun LuXin-Hua Shi
Nov 27, 2018·Journal of Cellular Biochemistry·Yunpeng SunHuanhuan Wu
Oct 28, 2019·Journal of Cellular and Molecular Medicine·Yang GaoPing Wang
Jun 9, 2019·Journal of Experimental & Clinical Cancer Research : CR·Nan LinRuiyun Xu
Apr 26, 2018·Breast Cancer Research and Treatment·Hidetaka ShimaItaru Endo
May 16, 2018·Cancer Biomarkers : Section a of Disease Markers·Bing ChenFeng Xu
Jan 4, 2020·Journal of Gastric Cancer·Haiyang YangZhengjiang Qian
Apr 26, 2020·Journal of Clinical Medicine·Dino BekricChristian Mayr
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Dec 6, 2018·Journal of Experimental & Clinical Cancer Research : CR·Yan WangLianxin Liu
Oct 8, 2016·Journal of Experimental & Clinical Cancer Research : CR·Shou-Hua WangZhi-Wei Quan
Sep 10, 2019·World Journal of Gastroenterology : WJG·Jun-Cheng WuMing-Yi Xu

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