Long Noncoding RNA HEIH Promotes Colorectal Cancer Tumorigenesis via Counteracting miR-939‒Mediated Transcriptional Repression of Bcl-xL

Cancer Research and Treatment : Official Journal of Korean Cancer Association
Chunhui CuiJinlong Yu

Abstract

Studies have found that long noncoding RNA HEIH (lncRNA-HEIH) is upregulated and facilitates hepatocellular carcinoma tumor growth. However, its clinical significances, roles, and action mechanism in colorectal cancer (CRC) remains unidentified. lncRNA-HEIH expression in CRC tissues and cell lines was measured by quantitative real-time polymerase chain reaction. Cell CountingKit-8, ethynyl deoxyuridine incorporation assay, terminal deoxynucleotidyl transferase dUTP nick end labeling staining, and nude mice xenografts assays were performed to investigate the roles of lncRNA-HEIH. RNA pull-down, RNA immunoprecipitation, chromatin immunoprecipitation, and luciferase reporter assays were performed to investigate the action mechanisms of lncRNA-HEIH. In this study, we found that lncRNA-HEIH is significantly increased in CRC tissues and cell lines. lncRNA-HEIH expression is positively associated with tumor size, invasion depth, and poor prognosis of CRC patients. Enhanced expression of lncRNA-HEIH promotes CRC cell proliferation and decreases apoptosis in vitro, and promotes CRC tumor growth in vivo. Whereas knockdown of lncRNA-HEIH inhibits CRC cell proliferation and induces apoptosis in vitro, and suppresses CRC tumor growth in viv...Continue Reading

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Citations

Sep 20, 2018·Journal of Cellular Biochemistry·Kegang JiaLian Xu
Feb 23, 2020·Cancer Biotherapy & Radiopharmaceuticals·Fangfang XieQian Sun
Sep 25, 2020·Asia-Pacific Journal of Clinical Oncology·Arash PoursheikhaniMohammad Amin Kerachian
May 31, 2019·Journal of Cellular Biochemistry·Yongbiao MaJianling Liu
May 13, 2020·BMC Bioinformatics·Qianqian YuanLin Gao
Jun 21, 2020·Signal Transduction and Targeted Therapy·Lei LiuShouping Xu
Dec 10, 2020·Cancer Cell International·Shengyang GeChuanyu Sun
Dec 29, 2020·Technology in Cancer Research & Treatment·Chuan ChengJianping Wang
Apr 21, 2021·Genetic Testing and Molecular Biomarkers·Xin ChenWenyan Yu
May 8, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kun WuBaofei Jiang
Jun 14, 2021·Journal of Experimental & Clinical Cancer Research : CR·Zhihui DouHong Zhang

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Methods Mentioned

BETA
Assay
PCR
transfections
pull-down
immunoprecipitation assay
immunoprecipitation
pulldown
RIP
ChIP

Software Mentioned

GraphPad Prism
TargetScan

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