Long noncoding RNA HOTAIR mediates the estrogen-induced metastasis of endometrial cancer cells via the miR-646/NPM1 axis

American Journal of Physiology. Cell Physiology
Yun-Xiao ZhouJun Chen

Abstract

LncRNA homeobox (HOX) transcript antisense intergenic RNA (HOTAIR) has been confirmed to be involved in the tumorigenic progression of endometrial carcinoma (EC). However, the molecular mechanisms of HOTAIR in EC are not fully elucidated. The expression of HOTAIR and miR-646 in human EC tissues was determined by qRT-PCR. The effect of miR-646 on EC cells was assessed by the cell viability, migration, and invasion using CCK-8 assays and transwell assays. RNA-binding protein immunoprecipitation assays and RNA pull-down assays were performed to explore the interaction between HOTAIR and miR-646. The regulation of miR-646 on nucleophosmin 1 (NPM1) was tested using luciferase reporter assays. MiR-646 expression was significantly decreased both in human EC tissues ( n = 23) and cell lines (Ishikawa and HEC-1-A) compared with the control. Moreover, miR-646 expression was negatively related to HOTAIR in human EC tissues ( n = 23). Our results also showed that miR-646 overexpression considerably attenuated the E2-promoted viability, migration, and invasion of Ishikawa and HEC-1-A cells in vitro. In addition, HOTAIR was confirmed to regulate the viability, migration, and invasion of EC cells through negative regulating miR-646. More impo...Continue Reading

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Citations

Jan 25, 2020·Journal of Cellular and Molecular Medicine·Ping LiXuelei Zhang
May 8, 2020·Journal of Cellular and Molecular Medicine·Yao LiuYang Zhao
Aug 21, 2019·Cancers·Rakesh KumarM Radhakrishna Pillai
Aug 30, 2018·Non-coding RNA·Mabel Yin-Chun YauChi-Ming Wong
Dec 15, 2018·Journal of Cellular Biochemistry·Qinyang XuYincheng Teng
Nov 26, 2020·Experimental and Molecular Pathology·Soudeh Ghafouri-FardMohammad Taheri
Jan 7, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Taruna RajagopalNageswara Rao Dunna
Apr 4, 2021·International Journal of Molecular Sciences·Roberto PiergentiliFederica Perelli
Apr 4, 2021·Cancers·Shailendra Kumar Dhar DwivediResham Bhattacharya
Jun 30, 2021·Journal of Orthopaedic Surgery and Research·Jipeng LuYing Xiong
Jun 15, 2021·International Journal of Endocrinology·Jiajie TuZhiying Yu
Aug 28, 2021·Healthcare·Anna Franca CavaliereFabrizio Signore
Nov 17, 2021·Critical Reviews in Food Science and Nutrition·Mina HomayoonfalBahman Yousefi

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Methods Mentioned

BETA
hysterectomy
PCR
Assay
transfection
protein assay
immunoprecipitation
RIP
pull-down

Software Mentioned

Genewiz
DIANA Tools

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