Long noncoding RNA LINC00336 inhibits ferroptosis in lung cancer by functioning as a competing endogenous RNA

Cell Death and Differentiation
Min WangBin Zhang

Abstract

The regulatory loop between long noncoding RNAs (lncRNAs) and microRNAs has a dynamic role in transcriptional and translational regulation, and is involved in cancer. However, the regulatory circuitry between lncRNAs and microRNAs in tumorigenesis remains elusive. Here we demonstrate that a nuclear lncRNA LINC00336 is upregulated in lung cancer and functions as an oncogene by acting as a competing endogenous RNA (ceRNAs). LINC00336 bound RNA-binding protein ELAVL1 (ELAV-like RNA-binding protein 1) using nucleotides 1901-2107 of LINC00336 and the RRM interaction domain and key amino acids (aa) of ELAVL1 (aa 101-213), inhibiting ferroptosis. Moreover, ELAVL1 increased LINC00336 expression by stabilizing its posttranscriptional level, whereas LSH (lymphoid-specific helicase) increased ELAVL1 expression through the p53 signaling pathway, further supporting the hypothesis that LSH promotes LINC00336 expression. Interestingly, LINC00336 served as an endogenous sponge of microRNA 6852 (MIR6852) to regulate the expression of cystathionine-β-synthase (CBS), a surrogate marker of ferroptosis. Finally, we found that MIR6852 inhibited cell growth by promoting ferroptosis. These data show that the network of lncRNA and ceRNA has an importan...Continue Reading

References

Jul 1, 2008·Cellular Signalling·Anke DollerWolfgang Eberhardt
Mar 23, 2010·Cell Cycle·Kotb AbdelmohsenMyriam Gorospe
Jun 19, 2010·Science·Demián CazallaJoan A Steitz
Dec 15, 2010·Genome Research·Kevin MyantIrina Stancheva
Mar 24, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yongguang TaoKathrin Muegge
May 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alice T ShawTyler Jacks
Aug 30, 2011·Nature·Mitchell GuttmanEric S Lander
May 29, 2012·Cell·Scott J DixonBrent R Stockwell
Jul 7, 2012·Biological Reviews of the Cambridge Philosophical Society·Shuang Liu, Yongguang Tao
Mar 19, 2013·Cell·Pedro J Batista, Howard Y Chang
Jul 6, 2013·Cell·Igor Ulitsky, David P Bartel
Aug 13, 2013·Cell Death and Differentiation·Z ZhangY-Y Mo
Aug 27, 2013·Lancet·Martin ReckKlaus F Rabe
Oct 10, 2013·Nature Reviews. Molecular Cell Biology·Sarah Geisler, Jeff Coller
Oct 25, 2013·Nature·Philipp Gut, Eric Verdin
Dec 19, 2013·Nature Chemical Biology·Scott J Dixon, Brent R Stockwell
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
Jan 21, 2014·Cell·Wan Seok YangBrent R Stockwell
Feb 22, 2014·Circulation Research·Kun WangPei-Feng Li
Mar 20, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Patrick M FordeRonan J Kelly
Sep 23, 2014·Science·Douglas R GreenGuido Kroemer
Jan 20, 2015·Nature Genetics·Matthew K IyerArul M Chinnaiyan
Jul 15, 2015·Molecular Cell·Minghui GaoXuejun Jiang
Nov 6, 2015·Nature Medicine·Maite Huarte
Jan 16, 2016·Cell Metabolism·Natalya N Pavlova, Craig B Thompson
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Mar 15, 2016·Nature Reviews. Genetics·Andrew P FeinbergAnita Göndör
Apr 14, 2016·Cancer Cell·Adam M Schmitt, Howard Y Chang
Jul 14, 2017·Cancer Research·Arunoday BhanSubhrangsu S Mandal

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Citations

Jun 13, 2020·Free Radical Research·Zhengjun WangLi Liu
Jun 25, 2020·Cells·Anna Martina BattagliaFlavia Biamonte
Aug 18, 2020·Autophagy·Xin ChenDaolin Tang
Sep 1, 2020·Cellular and Molecular Neurobiology·Ziyu WangWeirong Fang
Sep 24, 2020·Journal of Experimental & Clinical Cancer Research : CR·Xiaoming ZhangChaoshi Niu
Mar 4, 2020·Molecular Cancer·Xinyi ZhangYongguang Tao
May 7, 2020·Frontiers in Neuroscience·Xiaohua Song, Dingxin Long
Jun 14, 2020·Signal Transduction and Targeted Therapy·Ling ChenYongguang Tao
Sep 19, 2020·Apoptosis : an International Journal on Programmed Cell Death·Ying WangQianming Chen
Feb 6, 2020·American Journal of Respiratory Cell and Molecular Biology·Shunsuke MinagawaKazuyoshi Kuwano
Mar 13, 2020·Cellular & Molecular Biology Letters·Pengfei LiuLei Zhao
Dec 6, 2019·Molecular Cancer·Liuer HeGang Yin
Dec 23, 2020·Technology in Cancer Research & Treatment·Guoping XuXuening Zhang
Oct 2, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Zuli WangYongguang Tao
Jan 13, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xinyu GuHaihong Zhu
Dec 4, 2020·Cell Research·Daolin TangGuido Kroemer
Jan 9, 2021·Genomics & Informatics·Danbi SeoWanyeon Kim
Feb 10, 2021·Cell Death Discovery·Na JiangLei Shang
Feb 13, 2021·Oxidative Medicine and Cellular Longevity·Yue ChengXiaojing Liu
Feb 26, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shuzhen SunYapo Xu
Nov 14, 2020·International Journal of Molecular Sciences·Juan LiuZhaohui Feng
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Xi-Min HuKun Xiong
Apr 1, 2021·Seminars in Cancer Biology·Yanqing Liu, Wei Gu

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Methods Mentioned

BETA
reverse transcription PCR
xenograft
pulldowns
pulldown
pull-down
immunoprecipitation
RIP
transfection
Assay
PCR

Software Mentioned

ImageJ
Graphpad Prism
miRPathDB Microinspector
SPSS
RNAfold

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