Long noncoding RNA LINC00520 accelerates the progression of colorectal cancer by serving as a competing endogenous RNA of microRNA-577 to increase HSP27 expression

Human Cell
Xi-Han JinMing Chen

Abstract

The long noncoding RNA (lncRNA) LINC00520 is an important modulator of the oncogenicity of multiple human cancers. However, whether LINC00520 is involved in the malignant characteristics of colorectal cancer (CRC) has not been extensively studied until recently. Therefore, the present study aimed to detect LINC00520 expression in CRC and evaluate its clinical significance in patients with CRC. Functional experiments were conducted to test the biological roles and underlying mechanisms of LINC00520 in CRC progression. In this study, high-LINC00520 expression was verified in CRC tissues and cell lines, and this high expression was associated with patients' unfavorable clinicopathological parameters and shorter overall survival and disease-free survival. Functionally, interference of LINC00520 resulted in a significant decrease of CRC cell proliferation, migration, colony forming ability, and invasion. Mechanistically, LINC00520 functioned as a competing endogenous RNA by sponging microRNA-577 (miR-577) and thereby increasing heat shock protein 27 (HSP27) expression. Rescue experiments revealed that inhibiting miR-577 or restoring HSP27 could abrogate the effects of LINC00520 silencing on malignant phenotypes of CRC. LINC00520 fun...Continue Reading

References

Feb 26, 2009·Cell·Chris P PontingWolf Reik
Nov 18, 2011·Nature Reviews. Cancer·Kristine GlundeSabrina M Ronen
Apr 12, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·P GuoK Li
Feb 28, 2013·International Journal of Molecular Sciences·Nina Hauptman, Damjan Glavač
Mar 5, 2013·Bioorganic & Medicinal Chemistry Letters·Jeanette R McConnell, Shelli R McAlpine
Mar 19, 2013·Cell·Pedro J Batista, Howard Y Chang
Nov 15, 2013·Lancet·Hermann BrennerChristian Peter Pox
Mar 19, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Apr 28, 2016·The New England Journal of Medicine·H Gilbert Welch, Douglas J Robertson
Feb 6, 2017·Journal of Biochemical and Molecular Toxicology·Haiping JiangKan Jie
May 3, 2018·Experimental & Molecular Medicine·Yong WangXiandong Zeng
May 15, 2018·Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery·Yong Yan WuBin Quan Wang
Oct 5, 2018·Nucleic Acids Research·Zhenyu BaoDong Dong
Nov 2, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Fada XiaXinying Li
Dec 12, 2018·Journal of Cellular Physiology·Rasoul AbdollahzadehJavad Tavakkoly-Bazzaz
Feb 20, 2019·Non-coding RNA·Juliane C R FernandesSandra M Muxel
Sep 5, 2019·Cells·Yujing ChiJichun Yang
Oct 4, 2019·Oncology Letters·Yinghao Lv, Shuai Huang
Nov 5, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Feihu ChuHaiyan Miao
Dec 19, 2019·RNA Biology·Shengyun MaWendy Jia Men Huang

❮ Previous
Next ❯

Citations

Jun 7, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Sep 19, 2021·Cancer Cell International·Yu-Nan MaHao Zheng

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR
immunoprecipitation
Assay
electrophoresis
RIP

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.