Long noncoding RNA LINC00707 sponges miR-370-3p to promote osteogenesis of human bone marrow-derived mesenchymal stem cells through upregulating WNT2B

Stem Cell Research & Therapy
Bo JiaXiaoling Qiu

Abstract

Human bone marrow-derived mesenchymal stem cells (HBMSCs) are characterized by multiple differentiation potential and potent self-renewal ability, yet much remains to be elucidated on what determines these properties. Long-chain noncoding RNAs (lncRNAs) have been suggested to be involved in multiple biological processes under physiological and pathological conditions, including osteogenic differentiation. Alkaline phosphatase (ALP) activity assay, ALP staining, and Alizarin Red Staining were used for osteogenic potential detection. Western blot and qRT-PCR were used to examine the expression of LINC00707 and miR-370-3p. RNA-binding protein immunoprecipitation was used to detect the interaction between LINC00707 and RNA-induced silencing complex. Luciferase reporter assay was used to confirm the binding sites of miR-370-3p to LINC00707 and WNT2B. We demonstrated that LINC00707 expression was gradually increased in HBMSCs during consecutive osteogenic induction, and it could further positively regulate the osteogenic differentiation both in vitro and in vivo, whereas LINC00707 inhibition led to suppressed osteogenic differentiation. Thereafter, we inferred a predicted interaction between LINC00707 and miR-370-3p and then confirme...Continue Reading

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Citations

May 18, 2020·Cell Death & Disease·Xing DuQifa Li
Dec 8, 2020·Frontiers in Genetics·Suryaji PatilAirong Qian
Feb 20, 2021·Bioscience, Biotechnology, and Biochemistry·Xiequn ZouBing Wang
May 1, 2021·International Journal of Molecular Sciences·Cinzia AuriliaMaria Luisa Brandi
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Carmen LanzillottiFernanda Martini

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Methods Mentioned

BETA
PCR
Protein Assay
electrophoresis
xenografts
transfection
immunoprecipitation
Flow cytometry

Software Mentioned

CDS
R
TargetScan
EdgeR package
microT
Cufflinks

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