Abstract
Local anesthetics, including lidocaine (Lid), are widely used in clinical settings but new evidence suggested that they may induce strong neurological side-effects in human brains. In this work, we used an in vitro model to examine the functional modulations of a long non-coding RNA (lncRNA), LINC01347 on Lid-induced cytotoxicity in SH-SY5Y cells. SH-SY5Y cells were maintained in vitro and treated with Lid to induce cytotoxicity. Dynamic expression of LINC01347, in response to Lid treatment or lentivirus-mediated overexpression, was examined by quantitative real-time PCR. The effects of LINC01347 overexpression on Lid-induced cell death, LDH, caspase, and autophagy activities were evaluated. A potential downstream target of LINC01347, human microRNA-145-5p (hsa-miR-145-5p), was evaluated in SH-SY5Y cells. Hsa-miR-145-5p was subsequently upregulated to explore its functional correlation with LINC01347 in modulating Lid-induced SH-SY5Y cytotoxicity. Lid caused cell death and downregulated LINC01347 expression in SH-SY5Y cells in vitro. LINC01347 overexpression reduced Lid-induced cell death, LDH and caspase augmentation, and LC3B accumulation. Hsa-miR-145-5p was discovered to be closely affiliated with LINC01347. Its upregulation...Continue Reading
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