Long Noncoding RNA Lnc-SEMT Modulates IGF2 Expression by Sponging miR-125b to Promote Sheep Muscle Development and Growth

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Caihong WeiLixin Du

Abstract

Long noncoding RNAs (lncRNAs) are RNA transcripts that are more than 200 nt long but have little protein-coding potential. Within the last few years, thousands of lncRNAs have been identified and their functions in biological processes have begun to be understood. Although many studies havebegun to examine the functions of many noncoding RNAs, very little is known about the functions of long noncoding (lncRNA) function of livestock production and molecular mechanisms of their functions are still lackingrelated to livestock production. Expression of sheep enhanced muscularityTranscript lncRNA (lnc-SEMT) and miR-125b were examined in sheep using quantitative reverse-transcription polymerase chain reaction. Expression of Myod (myogenic determination factor), Myog (myoglobin) and Insulin-like growth factor 2 (IGF2)were examined by Western Blot.Luciferase reporter assays were performedto confirm the relationship between lnc-SEMT and miR-125b. Here, we identified a novel lnc-SEMT that promote sheep myoblast differentiation in vitro and enhanced sheep muscularity in vivo. Functional analyses showed that lnc-SEMT accelerates sheep myoblast differentiation in vitro. lnc-SEMT transgenic sheep exhibit a muscle hypertrophy phenotype charac...Continue Reading

Citations

Sep 19, 2019·The Journal of International Medical Research·Yao WangYu-Long Wang
Jul 22, 2020·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Hui Li, Junhong Jiang
Sep 5, 2019·American Journal of Physiology. Cell Physiology·Sébastien BonnetShizuka Uchida
Sep 30, 2020·Journal of Cellular Physiology·Liyi Wang, Tizhong Shan
Jan 30, 2021·Frontiers in Cell and Developmental Biology·Shanshan WangBo Zuo
Sep 22, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Xiaoli XuJiaxue Cao

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