Long noncoding RNA MALAT1 alleviates lipopolysaccharide-induced inflammatory injury by upregulating microRNA-19b in murine chondrogenic ATDC5 cells.

Journal of Cellular Biochemistry
Lin PanXingfu Li

Abstract

Osteoarthritis is the most frequent chronic bone-joint disease in middle-aged and older people worldwide. This study investigated the effects of long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on lipopolysaccharide (LPS)-induced murine chondrogenic ATDC5 cell inflammatory injury. Cell viability and apoptosis were assessed using cell counting kit-8 assay and annexin V-phycoerythrin (PE) staining, respectively. The expression levels of interleukin-1β (IL)-1β, IL-6, IL-8, tumor necrosis factor α (TNF-α), MALAT1, and microRNA-19b (miR)-19b were measured using quantitative reverse-transcription polymerase chain reaction. Enzyme-linked immunosorbent assay was conducted to detect the concentrations of IL-1β, IL-6, IL-8, and TNF-α in culture supernatant of ATDC5 cells. Expressions of key factors involved in cell apoptosis, proinflammatory response, Wnt/β-catenin, and nuclear factor κB (NF-κB) pathways were analyzed using Western blot analysis. We found that LPS treatment remarkably induced ATDC5 cell apoptosis and inflammatory injury. MALAT1 was upregulated in LPS-stimulated ATDC5 cells. Overexpression of MALAT1 significantly reversed the LPS-induced ATDC5 cell inflammatory injury, while suppression o...Continue Reading

References

Mar 20, 2010·Methods in Molecular Biology·Shahar Ish-Shalom, Amnon Lichter
Dec 7, 2011·Arthritis and Rheumatism·Tracey E SwinglerIan M Clark
Feb 9, 2012·International Journal of Molecular Sciences·Letizia Da SaccoAndrea Masotti
Apr 25, 2012·Medical Microbiology and Immunology·Christina BacheThomas Montag
Jun 7, 2012·Journal of Ethnopharmacology·Sung-Gyu LeeSang-Won Choi
Jun 12, 2012·Cell·Hans Clevers, Roel Nusse
Jun 12, 2012·Nucleic Acids Research·Michael P GantierBryan R G Williams
Jan 15, 2013·Sports Medicine and Arthroscopy Review·Kai Mithoefer
Mar 9, 2013·Current Reviews in Musculoskeletal Medicine·Sharon L Hame, Reginald A Alexander
Jun 25, 2013·Cancer Letters·Xuefei ShiYong Song
Aug 7, 2013·Molecular Cancer·Bastian Hoesel, Johannes A Schmid
Sep 6, 2013·Work : a Journal of Prevention, Assessment, and Rehabilitation·Berran YucesoyCecil M Burchfiel
Nov 1, 2013·Nature Reviews. Drug Discovery·Hui LingGeorge A Calin
Dec 5, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chih-Hung ChangSivasubramanian Savitha
Sep 10, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jinsoo SongEun-Jung Jin
Mar 20, 2015·Journal of Cellular and Molecular Medicine·Prasanth PuthanveetilSubrata Chakrabarti
Jan 2, 2016·Methods in Molecular Biology·Maria D Paraskevopoulou, Artemis G Hatzigeorgiou
Oct 30, 2016·Osteoarthritis and Cartilage·K MurataK Takayanagi
Dec 22, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Wei-Hui FangJian Zhang

❮ Previous
Next ❯

Citations

Jan 26, 2020·International Journal of Molecular Sciences·Zhi HeGuangyou Yang
Jan 12, 2020·Wiley Interdisciplinary Reviews. RNA·Siyu JiangMin Yang
Oct 3, 2020·Bioengineering & Translational Medicine·Louis J BornSteven M Jay
Aug 6, 2021·Archives of Biochemistry and Biophysics·Chuncha Bao, Chengqi He

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.