Long noncoding RNA MALAT1 in exosomes drives regenerative function and modulates inflammation-linked networks following traumatic brain injury

Journal of Neuroinflammation
Niketa A PatelPaula C Bickford

Abstract

Neuroinflammation is a common therapeutic target for traumatic brain injury (TBI) due to its contribution to delayed secondary cell death and has the potential to occur for years after the initial insult. Exosomes from adipose-derived stem cells (hASCs) containing the long noncoding RNA MALAT1 are a novel, cell-free regenerative approach to long-term recovery after traumatic brain injury (TBI) that have the potential to modulate inflammation at the genomic level. The long noncoding RNA MALAT1 has been shown to be an important component of the secretome of hASCs. We isolated exosomes from hASC containing or depleted of MALAT1. The hASC-derived exosomes were then administered intravenously to rats following a mild controlled cortical impact (CCI). We followed the rats with behavior, in vivo imaging, histology, and RNA sequencing (RNA Seq). Using in vivo imaging, we show that exosomes migrate into the spleen within 1 h following administration and enter the brain several hours later following TBI. Significant recovery of function on motor behavior as well as a reduction in cortical brain injury was observed after TBI in rats treated with exosomes. Treatment with either exosomes depleted of MALAT1 or conditioned media depleted of e...Continue Reading

References

Sep 1, 1996·Journal of Neurosurgery·S A BaldwinS W Scheff
May 1, 2002·Sports Medicine·Ian B Stewart, Don C McKenzie
Nov 4, 2004·Journal of Molecular Biology·Valeria de TurrisIrene Bozzoni
Nov 25, 2005·Stem Cells and Development·Martina VendrameAlison E Willing
Dec 17, 2005·Science·Shivendra Kishore, Stefan Stamm
Feb 1, 2007·Journal of Neurotrauma·Annadora J Bruce-KellerStephen W Scheff
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·S Kishore, S Stamm
May 23, 2009·Cell Death and Differentiation·A H Lund
Dec 24, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Bridgette D SempleM Cristina Morganti-Kossmann
Jan 8, 2010·Human Molecular Genetics·Shivendra KishoreStefan Stamm
Jul 14, 2010·Neurobiology of Disease·Bridgette D SempleM Cristina Morganti-Kossmann
Jul 28, 2012·Current Neurology and Neuroscience Reports·John MagnusonGeoffrey S F Ling
Dec 12, 2012·Neuron·Kaj BlennowHenrik Zetterberg
Dec 15, 2012·Lancet Neurology·Harvey Levin, Douglas Smith
Feb 1, 2013·Brain : a Journal of Neurology·Victoria E JohnsonWilliam Stewart
Jun 7, 2013·Oncology Letters·Yan GuoYongsheng Song
Aug 22, 2013·Journal of Neurotrauma·Kristen Dams-O'ConnorCynthia Harrison-Felix
Aug 22, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hongqi XinMichael Chopp
Nov 22, 2013·PloS One·Annika FendlerGeorge M Yousef
Feb 15, 2014·Biochemical and Biophysical Research Communications·Ziyi ZhaoChuanfang Wu
Apr 4, 2014·Journal of Neuroinflammation·Archana Gupta, Lynn Pulliam
Jul 19, 2014·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Lars Henning SchmidtRainer Wiewrodt
Jan 16, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Josh M MorgantiSusanna Rosi
Feb 24, 2015·Handbook of Clinical Neurology·Ann C Mckee, Daniel H Daneshvar

❮ Previous
Next ❯

Citations

Jun 17, 2019·Reviews in the Neurosciences·Mahasweta DasSubhra Mohapatra
Dec 7, 2019·Stem Cell Reviews and Reports·Jiaying YuanXuehong Liu
May 13, 2020·Critical Reviews in Clinical Laboratory Sciences·Siyuan ChenShenglin Huang
Mar 16, 2019·International Journal of Molecular Sciences·Jenni KarttunenAsla Pitkänen
Dec 25, 2019·Canadian Respiratory Journal : Journal of the Canadian Thoracic Society·Lu WangBin Xu
Jan 31, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Neel S SinghalDengke K Ma
Jun 27, 2020·Frontiers in Cell and Developmental Biology·Heather BranscomeFatah Kashanchi
Jul 2, 2020·International Journal of Nanomedicine·Yan WangQi Li
Apr 28, 2019·European Journal of Medical Research·Jinliang SunXuezhong Zhou
Feb 1, 2020·Biomicrofluidics·Zhijie MaHaiyan Li
Jul 1, 2020·Bone Research·Zhenhong NiLin Chen
Oct 3, 2020·Bioengineering & Translational Medicine·Louis J BornSteven M Jay
Nov 30, 2018·International Journal of Molecular Sciences·Karen KrukowskiSusanna Rosi
Jan 22, 2020·Neural Regeneration Research·Brooke BonsackCesar V Borlongan
Dec 16, 2020·Neural Regeneration Research·Meng-Shi YangBai-Yun Liu
Nov 16, 2020·International Immunopharmacology·Jiao MengWei Liu
Aug 23, 2019·Molecular Therapy. Nucleic Acids·Li Zhang, Handong Wang
Dec 19, 2020·Molecular Therapy. Nucleic Acids·Wenzhi YangAijun Ma
Dec 16, 2019·Neurochemistry International·Mario J BertogliatRaghu Vemuganti
Feb 12, 2021·Frontiers in Cell and Developmental Biology·Cristiana PistonoTarja Malm
Nov 17, 2020·Frontiers in Aging Neuroscience·Junfen FanYumin Luo
Dec 22, 2020·Frontiers in Neurology·Dana LengelRamesh Raghupathi
Dec 20, 2020·International Journal of Molecular Sciences·Leticia Moreno-GarcíaRosario Osta
Dec 17, 2020·Biology·Key-Hwan LimJae-Yeol Joo
Mar 9, 2021·Stem Cells International·Qian JinWenrong Xu
Apr 4, 2021·Biomolecules·Sarah A WalshThomas A Davis
Oct 21, 2020·Seminars in Cell & Developmental Biology·Ryan A SzetoCleber A Trujillo

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Methods Mentioned

BETA
antisense oligonucleotide
light scattering
RNA Seq
RNA-Seq
electrophoresis
PCR

Key Resources (RRID) Mentioned

SCR_014247
AB_2572212
SCR_003070

Software Mentioned

PRISM4
Ingenuity Pathway Analysis ( IPA )
GraphPad
TopHat
NIH Image
Living Image Software
HiSeq Sequencing Control
IPA
RNAcentral
BEDtools

Related Concepts

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Brain Injury & Trauma

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