Long noncoding RNA MAPKAPK5-AS1 promotes colorectal cancer progression by cis-regulating the nearby gene MK5 and acting as a let-7f-1-3p sponge.

Journal of Experimental & Clinical Cancer Research : CR
Ting YangJun Song

Abstract

Long noncoding RNAs (lncRNAs) are considered critical regulators in cancers; however, the clinical significance and mechanisms of MAPKAPK5-AS1 (hereinafter referred to as MK5-AS1) in colorectal cancer (CRC) remain mostly unknown. In this study, quantitative real-time PCR (qPCR) and western blotting were utilized to detect the levels of MK5-AS1, let-7f-1-3p and MK5 (MAPK activated protein kinase 5) in CRC tissues and cell lines. The biological functions of MK5-AS1, let-7f-1-3p and MK5 in CRC cells were explored using Cell Counting Kit-8 (CCK8), colony formation and transwell assays. The potential mechanisms of MK5-AS1 were evaluated by RNA pull-down, RNA immunoprecipitation (RIP), dual luciferase reporter assay, chromatin immunoprecipitation (ChIP) and bioinformatics analysis. The effects of MK5-AS1 and MK5 on CRC were investigated by a xenotransplantation model. We confirmed that MK5-AS1 was significantly increased in CRC tissues. Knockdown of MK5-AS1 suppressed cell migration and invasion in vitro and inhibited lung metastasis in mice. Mechanistically, MK5-AS1 regulated SNAI1 expression by sponging let-7f-1-3p and cis-regulated the adjacent gene MK5. Moreover, MK5-AS1 recruited RBM4 and eIF4A1 to promote the translation of MK5...Continue Reading

References

Jun 17, 1998·The EMBO Journal·L NewJ Han
Jun 29, 2000·Annual Review of Biochemistry·A C GingrasN Sonenberg
Jun 17, 2003·Molecular Biology of the Cell·Liguo NewJiahuai Han
May 3, 2006·Biological & Pharmaceutical Bulletin·Yukihiro AkaoTomoki Naoe
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jung-Chun LinWoan-Yuh Tarn
May 18, 2007·Nature Reviews. Cancer·Héctor PeinadoAmparo Cano
Feb 7, 2009·The New England Journal of Medicine·Jolien TolCornelis J A Punt
Sep 21, 2010·Journal of Dermatological Science·Yong LiGary J Fisher
Aug 2, 2011·Cell·Leonardo SalmenaPier Paolo Pandolfi
Feb 26, 2013·Nature Structural & Molecular Biology·Per JohnssonKevin V Morris
Sep 28, 2013·Cancer Discovery·Florian A Karreth, Pier Paolo Pandolfi
Oct 10, 2013·Nature Reviews. Molecular Cell Biology·Sarah Geisler, Jeff Coller
Nov 22, 2013·Breast Cancer Research and Treatment·Kristina P SørensenTorben A Kruse
Dec 29, 2015·Frontiers in Bioscience (Landmark Edition)·Maria PeranderOle-Morten Seternes
Feb 10, 2017·Molecular Metabolism·Giovanni Solinas, Barbara Becattini
Oct 24, 2017·Nucleic Acids Research·Rocco MazzoliniSílvia Canudas
Jul 18, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jinqiang ZhangTong Wu
Nov 22, 2018·Nature Reviews. Molecular Cell Biology·Anushka Dongre, Robert A Weinberg
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Mar 3, 2019·Nature Cell Biology·Oana-Diana Persa, Carien M Niessen
Jun 12, 2019·CA: a Cancer Journal for Clinicians·Kimberly D MillerRebecca L Siegel
Oct 22, 2019·Lancet·Evelien DekkerMichael B Wallace
Oct 28, 2019·Journal of Clinical Medicine·Hirofumi Noguchi
Oct 29, 2019·Journal of Cellular and Molecular Medicine·Ying ZhangWuyin Li

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Citations

Mar 31, 2021·Cell Death & Disease·Chen-Hua DongJun Song
Apr 23, 2021·Biochemical and Biophysical Research Communications·Hai-Rong ZhangZhong-Xue Fu
Jul 26, 2021·European Journal of Pharmacology·Chunyuan TangLushan Yu

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Methods Mentioned

BETA
PCR
transfection
coimmunoprecipitation
electrophoresis
immunoprecipitation
pull-down
ChIP
xenograft
RNA-Seq
transfections

Software Mentioned

DIANA Tools
DIANA
miRcode
SPSS
Ensembl Genome browser
GraphPad Prism
ImageJ

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