Long noncoding RNA MARL regulates antiviral responses through suppression miR-122-dependent MAVS downregulation in lower vertebrates

PLoS Pathogens
Qing ChuLei Zhang

Abstract

Increasing evidence suggests important roles for long noncoding RNAs (lncRNAs) as new gene modulators involved in various biological processes. However, the function roles of lncRNAs in lower vertebrates are still unknown. Here, we firstly identify a lncRNA, named MAVS antiviral-related lncRNA (MARL), as a key regulator for antiviral immunity in teleost fish. The results indicate that fish MAVS play essential roles in host antiviral responses and inhibition of Siniperca chuatsi rhabdovirus (SCRV) replication. miR-122 reduces MAVS expression and suppress MAVS-mediated antiviral responses, which may help viruses evade host antiviral responses. Further, MARL functions as a competing endogenous RNA (ceRNA) for miR-122 to control protein abundance of MAVS, thereby inhibiting SCRV replication and promoting antiviral responses. Our data not only shed new light on understanding the function role of lncRNA in biological processes in lower vertebrates, but confirmed the hypothesis that ceRNA regulatory networks exist widely in vertebrates.

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Citations

Dec 22, 2020·Developmental and Comparative Immunology·Xiaomeng RenYuena Sun
May 13, 2021·Developmental and Comparative Immunology·Wenjing DongTianjun Xu
Aug 15, 2021·Developmental and Comparative Immunology·Renjie ChangYuena Sun

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Methods Mentioned

BETA
transfection
immunoprecipitation
RIP
RNA-seq
PCR
pulldown
pull down
Protein Assay

Software Mentioned

CPC
Ensemble
Cuffmerge
CNCI
CPAT
SMRAT Library

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