Abstract
The long noncoding RNA (lnc) maternally expressed 3 (MEG3) is downregulated in many types of cancers. However, the relationship between lncRNA MEG3, microRNA-21 (miR-21) and chronic myeloid leukemia (CML) blast crisis is unknown. This study examined bone marrow samples from 40 CML patients and 10 healthy donors. Proliferation and apoptosis assays, real-time polymerase chain reaction (PCR), bisulfite sequencing PCR, Western blotting, luciferase assay, RNA pull-down, RNA immunoprecipitation (RIP), co-immunoprecipitation (CoIP) and Chromatin immunoprecipitation (ChIP) were performed. We found that MEG3 and PTEN expression were down-regulated, whereas, MDM2, DNMT1 and miR-21 were up-regulated in the accelerated and blast phases of CML. Treated with 5-azacytidine decreased the level of MDM2, DNMT1 and miR21, but increased the level of MEG3 and PTEN. Overexpression of MEG3 and silencing the expression of miR-21 inhibited proliferation and induced apoptosis. MEG3 overexpression and silencing the expression of miR21 influence the levels of MMP-2, MMP-9, bcl-2 and Bax. MEG3 was able to interact with MDM2 and EZH2. MDM2 could interact with DNMT1 and PTEN. MYC and AKT can interact with EZH2. ChIP-seq showed that the promoter of KLF4 and S...Continue Reading
References
Aug 10, 2000·British Journal of Haematology·C G Geary
Jan 10, 2002·Cell·V Ambros
Jun 1, 2002·Leukemia·A Janowska-WieczorekM Z Ratajczak
Nov 25, 2003·The New England Journal of Medicine·James G Herman, Stephen B Baylin
Dec 25, 2004·Blood·Michael DeiningerBrian J Druker
Jun 3, 2005·Annals of Hematology·Rüdiger HehlmannAndreas Hochhaus
Sep 29, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jian-Hua LuoGeorge K Michalopoulos
Feb 26, 2009·Cell·Chris P PontingWolf Reik
Feb 25, 2010·Cancer Research·Xun ZhangAnne Klibanski
Jun 25, 2010·Leukemia·Y ChenS Li
Jan 5, 2011·Cancer Research·Miao-Chih TsaiHoward Y Chang
Oct 31, 2012·Frontiers in Genetics·Keiko Tano, Nobuyoshi Akimitsu
Jan 29, 2013·Cell·Carla A KlattenhoffLaurie A Boyer
Mar 13, 2014·International Journal of Hematology·Juan R Alvarez-DominguezHarvey F Lodish
Mar 5, 2016·Oncotarget·Xiangming CaoWeisheng Shen
Oct 23, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Li ZhangLi Wang
Nov 18, 2016·The Journal of Biological Chemistry·Minoru TerashimaTakeshi Suzuki
Jan 7, 2017·Journal of Experimental & Clinical Cancer Research : CR·Jun ZhangTingting Yao
Feb 9, 2017·Cancer Chemotherapy and Pharmacology·Jing ZhangLi Li
Feb 14, 2017·Biomolecules & Therapeutics·Xiangyu ZhouZhiqiang Liu
Sep 14, 2017·Molecular Medicine Reports·Tao WangJinhua Dai
Feb 9, 2018·Cell Death & Disease·Meiyue LiuGuogui Sun
Feb 15, 2018·Molecular Cancer Therapeutics·Pritha DasguptaRajvir Dahiya
Citations
Mar 21, 2019·Cell Cycle·Xu XuLiangliang Wang
Jun 19, 2019·Journal of Cellular Biochemistry·Hui LiYanyan Sun
Nov 24, 2018·Journal of Hematology & Oncology·Nonthaphat Kent WongShea Ping Yip
Apr 10, 2020·Biomarkers in Medicine·Ancuta JurjIoana Berindan-Neagoe
May 3, 2020·International Journal of Laboratory Hematology·Yanan YuLi Jia
Jul 29, 2020·Technology in Cancer Research & Treatment·Chunling HeZhen Yang
Jun 6, 2019·Aging·Jun-Dan WangZi-Jie Long
Oct 3, 2020·Cancer Medicine·Chunyu ZhangGuoqing Shao
Nov 14, 2019·International Journal of Molecular Sciences·Fabien MuselliDidier Mary
Feb 23, 2019·Pathology Oncology Research : POR·Arwa Al-RugeebahNarasimha Reddy Parine
Aug 16, 2019·World Journal of Gastroenterology : WJG·Wei WangQing-Chang Li
Oct 1, 2019·PeerJ·Xiang SongZhiyong Yu
Sep 25, 2020·Asia-Pacific Journal of Clinical Oncology·Rana Muhammad UsmanFaiz Anwer
Oct 17, 2018·International Journal of Molecular Sciences·Mirco Di MarcoRosa Visone
Apr 9, 2019·Molecular Medicine Reports·Qing LuoJinbo Liu
Dec 30, 2020·International Journal of Laboratory Hematology·Mei JiangAiping Le
Jun 16, 2019·EBioMedicine·Weihao LiSong Zhao
Feb 11, 2021·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·L GaoJ Ding
Feb 19, 2021·Scandinavian Journal of Clinical and Laboratory Investigation·Wenjin Gao
May 5, 2021·Cell Death & Disease·Haiping SongHao Zhou
Jun 3, 2021·Cancers·Christian Boni, Claudio Sorio
Sep 4, 2021·Medicine·Hongjuan XueWei Gao