Long non‑coding RNA PVT1 promotes epithelial‑mesenchymal transition via the TGF‑β/Smad pathway in pancreatic cancer cells

Oncology Reports
Xingxing ZhangMin Xu

Abstract

Recent studies have revealed that overexpression of long non‑coding RNA (lncRNA) PVT1 is correlated with several types of cancer. However, its role in pancreatic cancer development remains to be clarified. In the present study, we found that PVT1 promoted the growth and the epithelial‑mesenchymal transition (EMT) of pancreatic cancer cells. We first determined that PVT1 was upregulated in pancreatic cancer tissues compared with adjacent normal tissues. Knockdown of PVT1 inhibited viability, adhesion, migration and invasion. Furthermore, PVT1 knockdown reduced the expression of mesenchymal markers including Snail, Slug, β‑catenin, N‑cadherin and vimentin, while it increased epithelial marker expression of E‑cadherin. Finally, knockdown of PVT1 inhibited the TGF‑β/Smad signaling, including p‑Smad2/3 and TGF‑β1 but enhanced the expression of Smad4. In contrast, overexpression of PVT1 reversed the process. These findings revealed that PVT1 acts as an oncogene in pancreatic cancer, possibly through the regulation of EMT via the TGF‑β/Smad pathway and PVT1 may serve as a potential target for diagnostics and therapeutics in pancreatic cancer.

Citations

Apr 21, 2019·International Journal of Molecular Sciences·Mila Gugnoni, Alessia Ciarrocchi
Jan 30, 2019·Journal of Cellular Physiology·Soudeh Ghafouri-Fard, Mohammad Taheri
Sep 23, 2020·Journal of Clinical Laboratory Analysis·Chuanhui XunJiangtao Chen
Sep 12, 2019·Frontiers in Oncology·Olorunseun O Ogunwobi, Adithya Kumar
Mar 3, 2020·Frontiers in Oncology·Onayemi Titilayo OnagoruwaOlorunseun O Ogunwobi
Apr 8, 2020·Frontiers in Oncology·Ruining Gong, Yueping Jiang

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