Abstract
Sevoflurane has been reported to promote learning and memory disabilities by promoting neuroinflammation and neuroapoptosis. However, the precise mechanism by which sevoflurane mediating neurotoxicity remains to be determined. Cell viability, reactive oxygen species (ROS) generation, inflammation and apoptosis were measured by cell counting kit-8 assay, ROS kit, ELISA, flow cytometry and western blot assay. The abundance of small nucleolar RNA host gene 1 (SNHG1) and microRNA-181b (miR-181b) was measured by quantitative real-time PCR in HT22 cells. The binding sites between miR-181b and SNHG1 were predicted by Starbase, and this combination was verified by dual-luciferase reporter assay, RNA immunoprecipitation and RNA-pull down assays. Sevoflurane treatment promoted ROS generation, inflammation and apoptosis while impeded the viability of HT22 cells via upregulating long noncoding RNA (lncRNA) SNHG1. MiR-181b was a direct target of SNHG1, and it was inversely regulated by SNHG1 in HT22 cells. The overexpression of miR-181b counteracted the neurotoxicity of sevoflurane treatment in HT22 cells. MiR-181b depletion abolished the inhibitory effects of SNHG1 intervention on the ROS generation, inflammation and apoptosis and the prom...Continue Reading
References
Mar 21, 2001·Apoptosis : an International Journal on Programmed Cell Death·H U SimonF Levi-Schaffer
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 6, 2003·Science·Chang-Zheng ChenDavid P Bartel
Sep 4, 2004·Genome Biology·Eric A MiskaH Robert Horvitz
Feb 13, 2009·Anesthesiology·Maiko SatomotoJunko Imaki
Mar 19, 2009·Anesthesiology·Robert T WilderDavid O Warner
Mar 19, 2011·Anesthesia and Analgesia·Charles DiMaggioGuohua Li
Nov 22, 2011·PLoS Genetics·Ashish LalJudy Lieberman
Jul 6, 2012·Current Pharmaceutical Design·Vesna Jevtovic-Todorovic
Jan 15, 2013·Anesthesiology·Hui ZhengZhongcong Xie
May 8, 2013·Glia·Emmette R HutchisonMark P Mattson
Feb 22, 2014·Molecular Cancer·Yogita K Adlakha, Neeru Saini
May 3, 2014·Anesthesiology·Guorong TaoZhongcong Xie
Jun 27, 2014·Stem Cells·Chi XuPing-Yee Law
Dec 3, 2014·Neurotoxicology·Mu-Huo JiJian-Jun Yang
Dec 9, 2014·Mutation Research. Reviews in Mutation Research·Xiaolei LiWeidong Han
Dec 23, 2015·Journal of Genetics·Mina KazemzadehAyla Valinezhad Orang
Feb 26, 2016·Molecular Neurobiology·Peixing WanYehong Zhuo
Mar 30, 2017·Brain Research Bulletin·Changhua ShiChuan Qin
Jul 4, 2017·Brain Research·Ligang ShanYi Zhang
Sep 8, 2017·Journal of Neurochemistry·Xiang LvHong Jiang
Jul 11, 2018·Frontiers in Immunology·Alireza TahamtanVahid Salimi
Jul 22, 2018·Neuroscience·Bingqing CaoQian Yang
Dec 7, 2018·Immunology Letters·Zhenyu WangZhao Yang
Apr 20, 2019·Cytokine·Yayuan LuMian Peng
May 9, 2019·Cell Biochemistry and Function·Yena ZhangZhiming Zhou
Aug 27, 2019·Biochemical and Biophysical Research Communications·Hui WangJie Chen
Sep 10, 2019·Experimental Cell Research·Chen QianXiaozheng He