Long noncoding RNA SNHG14 regulates ox-LDL-induced atherosclerosis cell proliferation and apoptosis by targeting miR-186-5p/WIPF2 axis

Human & Experimental Toxicology
Z TaoQ Jia

Abstract

To investigate the role of small nucleolus RNA host gene 14 (SNHG14) in the progression of atherosclerosis (AS), bioinformatics analysis, and other relevant experiments (cell counting kit-8, flow cytometry, quantitative real-time polymerase chain reaction, luciferase reporter, RNA immunoprecipitation, RNA pull-down, and western blot assays) were done. The current study revealed that SNHG14 level was high in the serum of AS patients and oxidized low-density lipoprotein (ox-LDL)-induced AS cell lines. Besides, we found that SNHG14 accelerated cell proliferation while inhibited cell apoptosis in ox-LDL-induced AS cell lines. Next, SNHG14 was confirmed to be a sponge for miR-186-5p in AS cells, and it was validated that SNHG14 regulated AS cell proliferation and apoptosis by sponging miR-186-5p. Moreover, we uncovered that WAS-interacting protein family member 2 (WIPF2) was a downstream target of miR-186-5p in AS cells. Finally, it was demonstrated that miR-186-5p modulated AS cell proliferation and apoptosis via targeting WIPF2. To conclude, our research disclosed that SNHG14 affected ox-LDL-induced AS cell proliferation and apoptosis through miR-186-5p/WIPF2 axis, which may provide a theoretical basis for the treatment and diagno...Continue Reading

References

Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Jul 19, 2011·Biochemical and Biophysical Research Communications·Yi WangXiaoqing Chen
Sep 29, 2011·Cardiovascular Drugs and Therapy·Sona MitraJawahar L Mehta
Jul 4, 2012·Lipids in Health and Disease·Hui YangSheng-Hua Zhou
Nov 28, 2012·Journal of Molecular Biology·Je-Hyun YoonMyriam Gorospe
Jun 27, 2014·Seminars in Cell & Developmental Biology·Je-Hyun YoonMyriam Gorospe
Mar 5, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ke-Jing WangZhi-Jian Chen
Dec 26, 2016·Atherosclerosis·Yvonne BaumerWilliam A Boisvert
Dec 27, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Quynh N DinhChristopher G Sobey
Mar 16, 2017·Trends in Molecular Medicine·Nadiya KhyzhaJason E Fish
Apr 28, 2017·Briefings in Bioinformatics·Yuwei ZhangQi Liao
Sep 12, 2017·Biochemical and Biophysical Research Communications·Meiling LiYanhui Ding
Nov 3, 2018·Journal of the American College of Cardiology·Zhengyi ZhangTamer Sallam
Nov 18, 2018·International Journal of Biological Sciences·Rui WangYulan Zhu
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lei JiangJinqian Zhang
Jan 15, 2019·Trends in Genetics : TIG·Laura B Chipman, Amy E Pasquinelli
Feb 23, 2019·Frontiers in Cellular and Infection Microbiology·Luka CulibrkScott J Tebbutt
Feb 27, 2019·Critical Reviews in Immunology·Jillian P Rhoads, Amy S Major
Mar 22, 2019·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zhiqiang ZhangMingyu Fan
Jun 30, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Pengfei JiaoGaofei Ren
Jul 13, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Hongjun ZhaiXiaoli Sun
Sep 9, 2019·Differentiation; Research in Biological Diversity·Renata Silvério de BarrosAlexandre Holthausen Campos
Dec 4, 2019·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Enhong XuJuntian Lang
Dec 4, 2019·Clinical and Experimental Pharmacology & Physiology·Jun WangQingguang Liu

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