Long Noncoding RNA uc002yug.2 Activates HIV-1 Latency through Regulation of mRNA Levels of Various RUNX1 Isoforms and Increased Tat Expression

Journal of Virology
Chen HuanWenyan Zhang

Abstract

The HIV-1 reservoir is a major obstacle to complete eradication of the virus. Although many proteins and RNAs have been characterized as regulators in HIV-1/AIDS pathogenesis and latency, only a few long noncoding RNAs (lncRNAs) have been shown to be closely associated with HIV-1 replication and latency. In this study, we demonstrated that lncRNA uc002yug.2 plays a key role in HIV-1 replication and latency. uc002yug.2 potentially enhances HIV-1 replication, long terminal repeat (LTR) activity, and the activation of latent HIV-1 in both cell lines and CD4+ T cells from patients. Further investigation revealed that uc002yug.2 activates latent HIV-1 through downregulating RUNX1b and -1c and upregulating Tat protein expression. The accumulated evidence supports our model that the Tat protein has the key role in the uc002yug.2-mediated regulatory effect on HIV-1 reactivation. Moreover, uc002yug.2 showed an ability to activate HIV-1 similar to that of suberoylanilide hydroxamic acid or phorbol 12-myristate 13-acetate using latently infected cell models. These findings improve our understanding of lncRNA regulation of HIV-1 replication and latency, providing new insights into potential targeted therapeutic interventions.IMPORTANCE The...Continue Reading

References

Oct 17, 2007·PLoS Pathogens·Xavier ContrerasB Matija Peterlin
Jul 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ahmad M KhalilJohn L Rinn
Dec 23, 2011·Nature·Stefanie JägerNevan J Krogan
Aug 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lea CunninghamPaul Liu
Nov 1, 2013·Nature Methods·Stephen A BustinJo Vandesompele
Nov 19, 2013·Cell·Debbie S Ruelas, Warner C Greene
Nov 22, 2013·Journal of Virology·Mel CampbellYoshihiro Izumiya
Jan 17, 2015·Nature Reviews. Cancer·Yoshiaki ItoLinda Shyue Huey Chuang
Feb 9, 2016·Molecular Cell·Roxana S RedisGeorge A Calin
Feb 15, 2017·Frontiers in Microbiology·Doreen Kamori, Takamasa Ueno
Jun 14, 2017·Clinical Chemistry·Stephen A Bustin, Carl T Wittwer

❮ Previous
Next ❯

Citations

Aug 4, 2018·Frontiers in Microbiology·Lipeng QiuKeping Chen
Nov 12, 2019·PloS One·Wim TrypsteenNadejda Beliakova-Bethell
Feb 19, 2021·Experimental Hematology·Takako Yokomizo-Nakano, Goro Sashida
Mar 16, 2021·Molecular Therapy. Nucleic Acids·Saikat Boliar, David G Russell
Sep 17, 2021·Essays in Biochemistry·Thomaz Lüscher-DiasHelder I Nakaya

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.