Long noncoding RNA UCA1 promotes cell growth, migration, and invasion by targeting miR-143-3p in oral squamous cell carcinoma

Cancer Medicine
Qingyun DuanHongbing Jiang

Abstract

The long noncoding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) is dysregulated in many types of tumors; however, its role in oral squamous cell carcinoma (OSCC) remains unclear. This study aims to determine the effect of lncRNA UCA1 on OSCC. Fifty-six paired OSCC and adjacent nontumorous tissues were collected and the levels of UCA1, miR-143-3p, and MYO6 in the tissues were evaluated by qRT-PCR. In in vitro experiments, cell viability, migration, and invasion were measured by, respectively, performing CCK-8, wound healing, and transwell assays. The target relationships among UCA1, miR-143-3p, and MYO6 were verified by dual-luciferase assay. Western blot and immunohistochemistry were carried out to determine the protein levels. Xenograft mouse model was established to explore the effects of UCA1 in vivo. Levels of UCA1 and MYO6 were increased significantly in OSCC, while the level of miR-143-3p was decreased compared with the adjacent nontumorous tissues. UCA1 promoted OSCC cell growth, migration, and invasion both in vitro and in vivo, while miR-143-3p reversed the progression. MYO6 was validated as a target for miR-143-3p, and MYO6 overexpression reversed the effects of miR-143-3p mimic on OSCC cells. LncRNA UCA1 con...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 9, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Sagar C PatelBhishamjit S Chera
Mar 19, 2014·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·M BloebaumP Kessler
Sep 26, 2015·Cancer Biotherapy & Radiopharmaceuticals·Hong WangZiang Yang
Oct 27, 2016·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Xinyu ZhangLiu Liu
May 18, 2017·Future Oncology·Su-Rui ShengXin-Hua Liang
Dec 9, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·An-Wei Wei, Li-Fang Li
Aug 22, 2018·Biochemical and Biophysical Research Communications·Fengting HuangHuanliang Liu
Sep 16, 2018·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shule XieZhaoyu Lin
Nov 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ben SangMian Wu
Nov 28, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Qiang LiYueshu Wang
Jan 18, 2019·Journal of Cellular Biochemistry·Mingfu CuiBin Song
Feb 10, 2019·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Kevin C LeeScott M Peters
Mar 3, 2019·Journal of Experimental & Clinical Cancer Research : CR·Shanshan DongYong Zeng
May 28, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Yu HouZhixing Liang

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Citations

Feb 2, 2021·Frontiers in Oncology·Yuming XuZhengjun Shang
Jun 3, 2021·International Journal of Molecular Sciences·Cing-Syuan LeiJing-Wen Shih
Jul 26, 2021·European Journal of Pharmacology·Chunyuan TangLushan Yu

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Methods Mentioned

BETA
transfection
xenograft

Software Mentioned

GraphPad Prism
Venny
ImageJ
GEO2R
starbase
GraphPad
Targetscan
Targetscan7

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