Long noncoding RNA UCA1 promotes cell proliferation, migration and invasion of human leukemia cells via sponging miR-126

European Review for Medical and Pharmacological Sciences
M-D SunS Yang

Abstract

Acute myeloid leukemia (AML) is a bone marrow malignancy. Long non-coding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) plays an important role in several cancers. However, the role of lncRNA UCA1 in AML remained unclear. LncRNA UCA1 expressions in different cell lines were determined by RT-PCR. In human myelogenous leukemia (ML) cell lines K562 and HL60, effects of lncRNA UCA1 knockdown on cell viability, migration, invasion, and apoptosis were assessed, respectively. Binding effects between lncRNA UCA1 and microRNA (miR)-126, and between miR-126 and RAC1 3'UTR were detected by RT-PCR and luciferase activity assay. Involvements of miR-126 and RAC1 in lncRNA UCA1-mediated cell bioactivities were assessed. We found that lncRNA UCA1 was upregulated in ML cell lines. Knockdown of lncRNA UCA1 inhibited cell viability, migration, invasion, and prompted apoptosis of ML cells in vitro. LncRNA UCA1 could bind with miR-126 and downregulate miR-126 expression. Simultaneously, the anti-growth and anti-metastasis actions of lncRNA UCA1 knockdown on ML cells were reversed by miR-126 suppression. RAC1 was a target gene of miR-126, and the anti-ML actions of miR-126 were abolished by RAC1 overexpression. Moreover, PI3K/AKT and JAK/STA...Continue Reading

Citations

Jul 25, 2019·Frontiers in Oncology·Michelle NgJan-Henning Klusmann
Nov 5, 2019·Frontiers in Oncology·Alina-Andreea ZimtaIoana Berindan-Neagoe
Nov 3, 2020·Frontiers in Oncology·Kaifee Arman, Tarik Möröy
Jan 10, 2021·Cancers·Irina A Elcheva, Vladimir S Spiegelman
May 1, 2021·Cancers·Ekaterina O GubernatorovaSergei A Nedospasov

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