Long noncoding RNA XIST promotes proliferation and invasion by targeting miR-141 in papillary thyroid carcinoma

OncoTargets and Therapy
Yawei XuJunling Wang

Abstract

The long noncoding RNA X-inactive specific transcript (XIST) was reported to play vital roles in tumor progression. In the present study, we determined the regulatory function of XIST in papillary thyroid carcinoma (PTC). XIST expression was determined in PTC tissues and cell lines by quantitative real-time polymerase chain reaction (PCR) (qRT-PCR). Cellular proliferation, migration, and invasion were measured using the Cell Counting Kit-8 (CCK-8) assay, wound-healing assay, and transwell invasion assay, respectively. Western blotting was used to determine protein expression. The downstream target miRNAs for XIST were identified by luciferase reporter assay and qRT-PCR. Relative expression of XIST was upregulated in PTC tissues and cell lines. High XIST expression was positively correlated with TNM stage and lymph node metastasis. Function assay demonstrated that knockdown of XIST significantly decreased cell proliferation, migration, and invasion in PTC cells. Moreover, we showed that the effects of XIST on PTC cell progression were mediated by miR-141. Our results demonstrated that XIST functioned as an oncogene in PTC progression by regulating miR-141, suggesting that XIST might be a promising therapeutic target for PTC trea...Continue Reading

Citations

Oct 15, 2019·Frontiers in Oncology·Xiaofeng DaiJianying Zhang
Oct 20, 2020·Fertility and Sterility·Tsai-Der ChuangOmid Khorram
Jan 8, 2021·Orphanet Journal of Rare Diseases·Berta Luzón-ToroSalud Borrego
Mar 16, 2021·Frontiers in Oncology·Omidvar RezaeiSoudeh Ghafouri-Fard
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Soudeh Ghafouri-FardSeyed Ali Mousavinejad
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Wenlun WangLingyun Zhu

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Methods Mentioned

BETA
dissection
PCR
scraping
Assay
transfection
flow cytometry

Software Mentioned

Starbase2
SPSS

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