Long Noncoding RNA XR007793 Regulates Proliferation and Migration of Vascular Smooth Muscle Cell via Suppressing miR-23b

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Ye-Xin WuFeng-Ting Liu

Abstract

BACKGROUND Long noncoding RNAs (lncRNAs) were identified as potential regulatory factor in vascular disease. However, the role of XR007793 in the regulation of neointima formation after vascular injury remains largely unknown. MATERIAL AND METHODS LncRNA expression levels were detected using real-time polymerase chain reaction (RT-PCR). In vivo and in vitro assay were performed in Sprague-Dawley rats and VSMCs. Cell Counting Kit-8 (CCK-8) assay, Transwell assay, and scratch wound healing assay were performed to detect cell proliferation and migration. Western blotting was used to detect protein expression. RESULTS The results of qRT-PCR indicated that XR007793 expression was significantly increased in the injured carotid artery of Sprague-Dawley rats and platelet-derived growth factor-BB induced rat aortic smooth muscle cells. Knockdown of XR007793 repressed the proliferation and migration of VSMC in vitro. The expression level of miR-23b was reduced in mouse carotid injured tissues and cell line. Bioinformatics analysis and luciferase reporter assay revealed that XR007793 directly bonds to miR-23b. Pearson correlation analysis showed that XR007793a and miR-23b were negatively correlated in carotid samples. Furthermore, bioinfo...Continue Reading

Citations

Sep 19, 2019·Cellular and Molecular Life Sciences : CMLS·Wen LiuBing Luo
Feb 6, 2020·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Xiaoying Jiang, Qilan Ning
Nov 16, 2020·Life Sciences·Yuchan YuanPeng Qu
Mar 6, 2021·Molecular Therapy. Nucleic Acids·Xi YangYonghong Li
Aug 22, 2021·Cardiovascular Toxicology·Mehran AmrovaniMozhdeh Sanei

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Methods Mentioned

BETA
Percutaneous
PCR
transfection

Software Mentioned

TargetScan Human
GraphPad Prism
SPSS

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