Long noncoding RNAs exchange during zygotic genome activation in goat

Biology of Reproduction
Mingtian DengYongjie Wan

Abstract

In mammals, their proper development during the early cleavage stages strongly relies on the gene products newly transcribed by zygotic genome activation (ZGA). Long noncoding RNAs (lncRNAs) have been characterized as key regulators of the ZGA process in mice and human. However, the ZGA stage has not yet been identified and epigenetic regulations of the ZGA process remain largely unknown in goats. Here, we show that ZGA occurred at the 8-cell stage in goats. During ZGA, trimethylation of H3K9 was dynamically changed but maintained strong staining in development arrested embryos. Using single-cell RNA sequencing, we identified 800 mRNAs and 250 lncRNAs as candidates of key molecules in goat preimplantation embryos. These mRNAs and lncRNAs were differentially expressed from 4- to 8-cell stage embryos and were strongly enriched in terms of retinoic acid receptor signaling pathway as well as signaling pathway regulating pluripotency of stem cells. In particular, we found that microinjection of siRNA against lnc_137 caused development arrest. Our results are consistent with the notion that lncRNAs play vital roles during ZGA, and the data presented here provide an excellent source for further ZGA lncRNA studies.

References

May 1, 1990·Molecular Reproduction and Development·N A TelfordG A Schultz
Mar 14, 2003·Glycobiology·Kelly G Ten HagenLawrence A Tabak
Sep 3, 2004·The EMBO Journal·John Peter McPhersonRazqallah Hakem
Mar 16, 2005·Developmental Biology·Fátima SantosWendy Dean
Sep 8, 2006·The FEBS Journal·Markus Sperandio
Sep 9, 2006·Cell·Kazuaki Ohtsubo, Jamey D Marth
Dec 22, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Srinivas R PallerlaKay Grobe
Feb 4, 2009·Nature Reviews. Genetics·Tim R MercerJohn S Mattick
Aug 25, 2009·Development·Wael Tadros, Howard D Lipshitz
Feb 10, 2012·Development·Muriel Rhinn, Pascal Dollé
Jan 5, 2014·Nature Protocols·Simone PicelliRickard Sandberg
Jun 19, 2014·Genes & Development·Michael J HerrigesEdward E Morrisey
Aug 26, 2014·Annual Review of Cell and Developmental Biology·Miler T LeeAntonio J Giraldez
Sep 5, 2014·BMC Genomics·Zongliang JiangXiuchun Cindy Tian
Jun 20, 2016·Glycoconjugate Journal·Kazuko Handa, Sen-Itiroh Hakomori
Sep 23, 2016·Human Reproduction Update·Julien BouckenheimerJohn De Vos
Jan 15, 2017·Current Opinion in Genetics & Development·Máté PálfyNadine L Vastenhouw
Apr 22, 2017·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Rosa KarlicPetr Svoboda
Dec 14, 2017·Seminars in Cell & Developmental Biology·Petr Svoboda
Jan 13, 2018·Biochimica Et Biophysica Acta. Molecular Cell Research·Xiaofeng LiHsiao Chang Chan

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Citations

Jan 15, 2019·Reproduction, Fertility, and Development·Mingtian DengFeng Wang
Nov 21, 2019·Zygote : the Biology of Gametes and Early Embryos·Mingtian DengFeng Wang
Feb 20, 2020·DNA and Cell Biology·Mingtian DengFeng Wang
Oct 30, 2020·Frontiers in Cell and Developmental Biology·Mingtian DengFeng Wang
Mar 20, 2021·Molecular Therapy. Nucleic Acids·Mingtian DengFeng Wang
Jul 1, 2021·Biology of Reproduction·Mingtian DengFeng Wang
Jul 31, 2020·Theriogenology·Mingtian DengYongjie Wan

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