Long period fiber grating based sensor for the detection of triacylglycerides

Biosensors & Bioelectronics
Anjli BaliyanEnakshi K Sharma

Abstract

In this paper, stable, label free enzyme based sensor using long period fiber grating (LPG) is described for the detection of triacylglycerides. A stable covalent binding technique for lipase enzyme immobilization on an optical fiber is reported. An active and stable attachment of the functional group of the enzyme on the fiber surface is achieved using this method. Enzyme immobilization is confirmed by Scanning Electron Microscopy (SEM) and Raman Spectroscopy. The stability is confirmed by lipase p-nitrophenyl palmitate (PNP) assay. In contrast to widely used amperometric based biosensor, where a number of enzymes are required, only one enzyme, namely, lipase is required in our sensor. The sensor shows optimum response within one minute at a temperature of 37°C and pH of 7.4. The sensor is based on the shift in resonance wavelength of the LPG transmission spectrum due to the interaction of triacylglycerides with the enzyme. The biosensor is highly specific towards triacylglycerides and is unaffected by the presence of many other interfering substances in serum. Interaction between the bio-molecules and the long period grating surface is also modeled theoretically using a four layer model for the LPG fiber with the bio-recognit...Continue Reading

References

Jan 27, 2009·Chemical Society Reviews·Ulf HanefeldEdmond Magner
Nov 6, 2012·Journal of Biophotonics·Francesco ChiavaioliFrancesco Baldini

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Citations

Mar 6, 2019·The Review of Scientific Instruments·Siddharth KaushikRavindra K Sinha
Sep 21, 2019·Biosensors & Bioelectronics·Yangyang ChenXiaohong Zhou

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