PMID: 2506545Sep 1, 1989Paper

Long-range intramolecular electron transfer in azurins

Proceedings of the National Academy of Sciences of the United States of America
O Farver, Israel Pecht

Abstract

The Cu(II) sites of azurins, the blue single copper proteins, isolated from Pseudomonas aeruginosa and Alcaligenes spp. (Iwasaki) are reduced by CO2- radicals, produced by pulse radiolysis, in two distinct reaction steps: (i) a fast bimolecular phase, at the rates (5.0 +/- 0.8) x 10(8) M-1.s-1 (P. aeruginosa) and (6.0 +/- 1.0) x 10(8) M-1.s-1 (Alcaligenes); (ii) a slow unimolecular phase with specific rates of 44 +/- 7 s-1 in the former and 8.5 +/- 1.5 s-1 for the latter (all at 298 K, 0.1 M ionic strength). Concomitant with the fast reduction of Cu(II), the single disulfide bridge linking cysteine-3 to -26 in these proteins is reduced to the RSSR- radical ion as evidenced by its characteristic absorption band centered at 410 nm. This radical ion decays in a unimolecular process with a rate identical to that of the slow Cu(II) reduction phase in the respective protein, thus clearly suggesting that a long-range intramolecular electron transfer occurs between the RSSR- radicals and the Cu(II) site. The temperature dependence of the internal electron transfer process in both proteins was measured over the 4 degrees C to 42 degrees C range. The activation parameters derived are delta H* = 47.5 +/- 4.0 and 16.7 +/- 1.5 kJ.mol-1; and...Continue Reading

References

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Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·O Farver, I Pecht

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Citations

Mar 22, 2012·Inorganic Chemistry·Alexey PotapovDaniella Goldfarb
May 9, 2012·Journal of the American Chemical Society·Kyle M LancasterHarry B Gray
May 29, 2000·European Journal of Biochemistry·O FarverI Pecht
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·O Farver, I Pecht
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K V MikkelsenO Farver
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·O FarverJ Ulstrup
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·O FarverI Pecht
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Qijin ChiJens Ulstrup
Jul 28, 2011·The FEBS Journal·Ole FarverIsrael Pecht
Sep 15, 2004·Journal of Molecular Biology·Anastasia V ZhuravlevaVladislav Yu Orekhov
Aug 25, 2015·Biochimica Et Biophysica Acta·Parisa Hosseinzadeh, Yi Lu
Sep 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Cunlan GuoDavid Cahen
Jan 28, 2020·Chemistry : a European Journal·Lingling ZhangUlrich Schwaneberg
Feb 8, 2011·Journal of the American Chemical Society·Lior SepunaruDavid Cahen
Feb 12, 2019·Chemical Reviews·Kazuo Kobayashi
Nov 4, 2020·Journal of the American Chemical Society·Jerry A FereiroDavid Cahen

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