PMID: 8938429Nov 1, 1996Paper

Long-range map of a 3.5-Mb region in Xp11.23-22 with a sequence-ready map from a 1.1-Mb gene-rich interval

Genome Research
D SchindelhauerA Meindl

Abstract

Most of the yeast artificial chromosomes (YACs) isolated from the Xp11.23-22 region have shown instability and chimerism and are not a reliable resource for determining physical distances. We therefore constructed a long-range pulsed-field gel electrophoresis map that encompasses approximately 3.5 Mb of genomic DNA between the loci TIMP and DXS146 including a CpG-rich region around the WASP and TFE-3 gene loci. A combined YAC-cosmid contig was constructed along the genomic map and was used for fine-mapping of 15 polymorphic microsatellites and 30 expressed sequence tags (ESTs) or sequence transcribed sites (STSs), revealing the following order: tel-(SYN-TIMP)-(DXS426-ELK1)-ZNF(CA) n-L1-DXS1367-ZNF81-ZNF21-DXS6616- (HB3-OATL1pseudogenes-DXS6950)-DXS6949-DXS694 1-DXS7464E(MG61)-GW1E(EBP)- DXS7927E(MG81)-RBM- DXS722-DXS7467E(MG21)-DXS1011E-WASP-DXS6940++ +-DXS7466E(MG44)-GF1- DXS226-DXS1126-DXS1240-HB1- DXS7469E-(DXS6665-DXS1470)-TFE3-DXS7468E-+ ++SYP-DXS1208-HB2E-DXS573-DXS1331- DXS6666-DXS1039-DXS 1426-DXS1416-DXS7647-DXS8222-DXS6850-DXS255++ +-CIC-5-DXS146-cen. A sequence-ready map was constructed for an 1100-kb gene-rich interval flanked by the markers HB3 and DXS1039, from which six novel ESTs/STSs were isolated, thus increas...Continue Reading

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Citations

Mar 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D Schindelhauer
Feb 5, 1999·Gene·K L StoddartN T Bech-Hansen
Jan 2, 2003·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Gail E HermanAida B Metzenberg
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·L CarrelH F Willard
Sep 17, 2013·Biochimica Et Biophysica Acta·Javier CañuetoRogelio González-Sarmiento
Nov 25, 2003·The Journal of Investigative Dermatology·Neil V WhittockPeter T Clayton
Sep 2, 2011·Journal of Molecular Signaling·Anne Kristin AksaasBjørn Steen Skålhegg
Oct 16, 2010·American Journal of Medical Genetics. Part a·Larissa V FurtadoAlan F Rope
Jan 27, 2005·Molecular Genetics and Metabolism·Lina S Correa-Cerro, Forbes D Porter
Jun 13, 1998·American Journal of Human Genetics·K M BoycottN T Bech-Hansen
May 26, 1999·Journal of Molecular Biology·M F BauerS Hofmann
Oct 20, 2000·American Journal of Medical Genetics·S IkegawaY Nakamura

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