Long-range multilocus haplotype phasing of the MHC.

Proceedings of the National Academy of Sciences of the United States of America
Zhen GuoE W Petersdorf

Abstract

Haplotypes are a powerful tool for identifying the genetic basis of common complex diseases. Disease-association mapping requires molecular methods for haplotyping biallelic SNP variation and highly complex polymorphisms. We developed a method for phasing HLA-A, HLA-B, and HLA-DRB1 alleles on chromosome 6 in unrelated individuals. This method uses the highly polymorphic HLA-B locus to discriminate the two HLA haplotypes in heterozygous individuals and its ideal location 1.4 Mbp telomeric to HLA-DRB1 and 1.2 Mbp centromeric to HLA-A to capture 2-Mbp-long genomic DNA. Genomic DNA representing a single HLA-B-captured haplotype is genotyped for HLA-A and HLA-DRB1 alleles and linkage to HLA-B is established. Proof of principle was established in a large blinded study of phase-known samples. Availability of an efficient method for MHC haplotype phase determination will facilitate the mapping of causative MHC-resident genes in many human diseases and has the potential to be broadened to other polymorphic gene complexes.

References

Feb 11, 1988·Nucleic Acids Research·S A MillerH F Polesky
Jan 1, 1995·Molecular Biology and Evolution·T Ota, M Nei
May 13, 1999·Immunological Reviews·M Carrington
Jul 11, 2000·Nature Biotechnology·A T WoolleyC M Lieber
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·C M DrysdaleS B Liggett
Oct 12, 2000·The New England Journal of Medicine·S K TakemotoJ M Cecka
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·X B ZhongD C Ward
Jul 28, 2001·Progress in Biophysics and Molecular Biology·J H HafnerC M Lieber
Dec 26, 2001·The New England Journal of Medicine·E W PetersdorfC Anasetti
Jul 26, 2002·Nucleic Acids Research·Ester InbarAriel Darvasi
Sep 26, 2002·American Journal of Human Genetics·Michael CullenMary Carrington
Nov 27, 2002·BioTechniques·Jacob OdebergMathias Uhlén
May 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Robi D MitraGeorge M Church
Jun 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chunming Ding, Charles R Cantor
Apr 27, 2004·Human Mutation·Pui-Yan Kwok, Ming Xiao
Sep 2, 2004·Cold Spring Harbor Symposia on Quantitative Biology·P Y Kwok, M Xiao
Oct 23, 2004·Genomics·Chang-En YuGerard D Schellenberg
Dec 2, 2004·Nature Reviews. Genetics·Roger HortonStephan Beck
Jan 8, 2005·Nucleic Acids Research·James D HurleyJohn E Landers
Apr 2, 2005·Human Heredity·Ma'ayan FishelsonDan Geiger
Aug 16, 2005·Methods in Molecular Biology·Matthias Wjst, Dirk van den Boom
Oct 7, 2005·Nature
Oct 7, 2005·Nature
Feb 24, 2006·Nature Genetics·Kun ZhangGeorge M Church

❮ Previous
Next ❯

Citations

Dec 21, 2010·Nature Biotechnology·H Christina FanStephen R Quake
Oct 8, 2009·Briefings in Functional Genomics & Proteomics·Kalim U Mir
May 31, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Johannes PröllChristian Gabriel
Oct 4, 2008·Current Opinion in Hematology·Effie W Petersdorf, John A Hansen
Aug 5, 2011·Genome Research·Eun-Kyung SukMargret R Hoehe
Mar 5, 2011·BMC Bioinformatics·Christine LoVineet Bafna
Mar 24, 2007·PLoS Medicine·Effie W PetersdorfZhen Guo
Mar 6, 2010·Annals of the New York Academy of Sciences·Vadim YuferovMary Jeanne Kreek
Mar 1, 2010·Molecular Ecology Resources·W Babik
Jul 28, 2006·Current Opinion in Immunology·Effie W Petersdorf
Oct 4, 2011·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Florence BettensJean-Marie Tiercy
Jul 21, 2007·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Mari MalkkiUNKNOWN International Histocompatibility Working Group in Transplantation
Jun 22, 2013·Journal of the American Society of Nephrology : JASN·Joseph R LeventhalJoshua Miller
Nov 7, 2015·BMC Genomics·Siddarth SelvarajBing Ren
Aug 15, 2017·Analytical Chemistry·Tsz Wing FanI-Ming Hsing
Sep 28, 2021·International Journal of Immunogenetics·Susanne SeitzAlexander H Schmidt
Dec 28, 2021·International Journal of Immunogenetics·Brian D Tait

❮ Previous
Next ❯

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