Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects.

Developmental Cell
James M HarrisPaola Arlotta

Abstract

Axons connect neurons together, establishing the wiring architecture of neuronal networks. Axonal connectivity is largely built during embryonic development through highly constrained processes of axon guidance, which have been extensively studied. However, the inability to control axon guidance, and thus neuronal network architecture, has limited investigation of how axonal connections influence subsequent development and function of neuronal networks. Here, we use zebrafish motor neurons expressing a photoactivatable Rac1 to co-opt endogenous growth cone guidance machinery to precisely and non-invasively direct axon growth using light. Axons can be guided over large distances, within complex environments of living organisms, overriding competing endogenous signals and redirecting axons across potent repulsive barriers to construct novel circuitry. Notably, genetic axon guidance defects can be rescued, restoring functional connectivity. These data demonstrate that intrinsic growth cone guidance machinery can be co-opted to non-invasively build new connectivity, allowing investigation of neural network dynamics in intact living organisms.

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Citations

Nov 5, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Robin Hiesinger
Nov 5, 2020·Physical Biology·Céline BraïniCatherine Villard
Jan 16, 2021·Tissue Engineering. Part C, Methods·Renata Vieira de SáR Jeroen Pasterkamp
Sep 25, 2020·Neuron·Ashley Del DossoGiorgia Quadrato
Dec 12, 2020·Cells·Marie-José BoueidMarcel Tawk
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Kazuhide AsakawaKoichi Kawakami
May 22, 2021·Journal of Molecular Biology·Vishnu V KrishnamurthyKai Zhang
Dec 17, 2021·Frontiers in Cell and Developmental Biology·Sarah A Neely, David A Lyons
Nov 15, 2021··James M. HarrisAndy Yu-Der Wang

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Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.