Long-range repression by multiple polycomb group (PcG) proteins targeted by fusion to a defined DNA-binding domain in Drosophila.

Genetics
R R RosemanP K Geyer

Abstract

A tethering assay was developed to study the effects of Polycomb group (PcG) proteins on gene expression in vivo. This system employed the Su(Hw) DNA-binding domain (ZnF) to direct PcG proteins to transposons that carried the white and yellow reporter genes. These reporters constituted naive sensors of PcG effects, as bona fide PcG response elements (PREs) were absent from the constructs. To assess the effects of different genomic environments, reporter transposons integrated at nearly 40 chromosomal sites were analyzed. Three PcG fusion proteins, ZnF-PC, ZnF-SCM, and ZnF-ESC, were studied, since biochemical analyses place these PcG proteins in distinct complexes. Tethered ZnF-PcG proteins repressed white and yellow expression at the majority of sites tested, with each fusion protein displaying a characteristic degree of silencing. Repression by ZnF-PC was stronger than ZnF-SCM, which was stronger than ZnF-ESC, as judged by the percentage of insertion lines affected and the magnitude of the conferred repression. ZnF-PcG repression was more effective at centric and telomeric reporter insertion sites, as compared to euchromatic sites. ZnF-PcG proteins tethered as far as 3.0 kb away from the target promoter produced silencing, ind...Continue Reading

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Citations

Jul 29, 2004·Genetics·Amanda J Moehring, Trudy F C Mackay
May 21, 2005·The Journal of Biological Chemistry·Chongwoo A KimJames U Bowie
Mar 15, 2002·Current Opinion in Genetics & Development·Jeffrey A Simon, John W Tamkun
Jul 5, 2003·Experimental Hematology·Julie Lessard, Guy Sauvageau
Mar 31, 2010·Molecular and Cellular Biology·Judith A Kassis, James A Kennison
Jun 13, 2015·Genes & Development·Hyuckjoon KangMitzi I Kuroda

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