Long-size isoelectricfocusing (IEF) in flexible silicone tubes: an application to semi-preparative fractionation of proteins

International Journal of Biological Macromolecules
A Galat, Robert Thai

Abstract

Soluble proteins extracted from porcine brains were subjected to a series of optional fractionation steps on various chromatographic media including a novel device for semi-preparative isoelectrofocusing (IEF) carried in a flexible silicone tube. The dimensions of the IEF granulated gel beds can be varied from 40 to 75 cm (length) and 0.4-1.6 cm (diameter) which are dependent on the protein's concentration. An average optimal focalisation time of proteins is dependent of the tube length, its diameter and complexity of proteins' mixtures but it is usually reached during 15,000-30,000 Vh. A series of sequential protein's fractionation techniques including semi-preparative IEF carried in the flexible silicone tube with the following dimensions: 75 cm in length and 1.6 cm in diameter permitted for observation and partial characterisation of several proteins whose expression levels are specifically high in the brain.

References

May 15, 1980·Analytical Biochemistry·B P Voris, D A Young
Jun 15, 1996·Archives of Biochemistry and Biophysics·A GalatS Rivière
Oct 25, 1996·Science·A GoffeauS G Oliver
Sep 5, 1997·Science·F R BlattnerY Shao
Jun 5, 1999·Journal of Molecular Biology·M R WilkinsD F Hochstrasser
Dec 11, 1999·Nucleic Acids Research·D L WheelerB A Rapp
Jun 24, 2000·Nature·A Pandey, M Mann
Jul 20, 2000·Archives of Biochemistry and Biophysics·M LeclercqA Galat
Feb 22, 2001·Science·J C VenterX Zhu
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium

❮ Previous
Next ❯

Citations

Jul 8, 2014·Biochemical and Biophysical Research Communications·Andrzej Galat, Robert Thai
Jun 1, 2014·International Journal of Biological Macromolecules·Andrzej GalatEnrico A Stura

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Control & Proteolysis

Key regulators of cell cycle, including cyclins, cyclin dependent kinase inhibitors, DNA replication factors, are controlled by proteolysis. Discover the latest research on cell cycle control and proteolysis.